Washwormsoffplates,putonice. Washwormsinwater. Mixandequalvolumeofwormsandsucrose. Spin5min. DrawofffloatingwormswithapasteurPipette. Washwormsfreeofanysucrose.Ifwormsaretobekeptalive,beverycarefultouseaseptictechniqueCleaningwormsbyfloatingonsucrose
Havecold70%sucroseinwater.Typicallyweuse2-1010-cmplatesofcrowdedorstarvedwormsForthismanyworms,usea15mlorange-capcentrifugetubeWashoffwithM9,S-basal,orwaterBesuretogrowwormsonagaroseplateswhenmakingDNAtobecut
Thisisoptional,itmakesthemmorebuoyantFora15mltube,spininclinicalcentrifugeat#3or4
30-35%sucrosefinal,7-10mltotalKeepcold,wormswillthrash
fora15mltube,spininclinicalcentrifugeat#6or7
Bacteria,deadwormsandotherdensethingswillbeonthebottom
