Description
Product Highlights
- Sensitive – incorporates MyTaq HS DNA Polymerase that exhibits increased affinity for DNA, thereby improving amplification of even limiting amounts of template
- Efficient – novel buffer system maximizes efficiency of PCR amplification, delivering improved yield of any PCR product
- Robust – reliable amplification in the presence of inhibitors and with even the most challenging DNA targets
- Flexible – ideal for amplifying any target up to 5 kb from DNA extracted from human, animal and plant samples
- Convenient – an all-in-one-tube mastermix and red dye, that improves the speed, convenience and accuracy of PCR set-up
- Fast – developed to give sensitive, reproducible and robust amplification of a broader range of targets under fast thermal cycling condition
Product Description
MyTaq™ HS Red Mix is recommended for PCR assays containing complex and low copy number targets as well as multiplex PCR. MyTaq HS Red Mix is comprised of MyTaq HS DNA Polymerase and a novel buffer system that deliver very high yield PCR amplification over a wide range of PCR templates. MyTaq HS has an increased affinity for DNA, enabling reliable amplification from even very low amounts of template. MyTaq HS has been developed to give more robust amplification than other commonly-used polymerases allowing it to perform well with challenging templates and in the presence of PCR inhibitors. Furthermore, the highly efficient nature of MyTaq HS means it gives excellent results under fast PCR conditions. MyTaq HS does not possess polymerase activity during the reaction set-up, thereby reducing the non-specific amplification that can hinder PCR assays from the start.
The product is supplied as a mastermix that requires the addition of only template, primers and water, thereby reducing the number of pipetting steps during PCR set-up for improved speed, throughput and assay reproducibility. The inclusion of dNTPs, MgCl2 and enhancers at optimal concentrations, helps eliminate the need for optimization, thereby saving time and ensuring comparable efficiencies for annealing and extension of all primers in the reaction, this makes MyTaq HS Red Mix ideal for multiplex PCR.
MyTaq HS Red Mix, includes a 5x MyTaq Red Reaction Buffer that increases the visual contrast between the reagent and the reaction vessel for improved convenience and to improve pipetting accuracy. The red dye also enables samples to be loaded directly on to a gel after the PCR eliminating the need to add loading buffer.
Applications
- Fast PCR
- Multiplex PCR
- Genotyping
- Complex templates (e.g. GC-rich)
- Colony PCR
- Low copy number PCR assays
- High-throughput assays with prolonged PCR set-up
Introduction to MyTaq
Overview, features and benefits of the MyTaq product familyPCR Enzyme Guide
Download the PCR Enzyme Guide with detailed product descriptions and performance data to help you choose the best product for your researchPCR Selection Chart
Select the best reagent for your researchApplication Note
MyTaq™ HS Red Mixebiomall.com
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蚂蚁淘主营海外试剂抗体,进口科研仪器,海外试剂耗材等所有行业相关用品进口。蚂蚁淘全球直采,正品保障,为科研献力!如需商业合作,请联系cooperation@ebiomall.com 。
德国MoBiTec公司于1987年由Stephan Diekmann 教授创立,由于和Max-Planck协会(德国处于领先地位的研究中心)有着密切的关系,这使得本公司与很多具有创造性的科学家有着亲密的合作。另外,MoBiTec 拥有来自欧洲和北美的研究人员,包括法国的Pasteur 研究中心和荷兰的Vrije大学的科学家。MoBiTec公司能提供独一无二的高质量产品,从而简化实验设计,而留出更多更宝贵的时间用于实际的研究。
产品包括: 双杂交和单杂交系统——体内蛋白-蛋白和DNA-蛋白关系,噬菌体展示系统——筛选特殊的多肽配体和选择具有特异性结合特性的新的蛋白质,Lamda PS基因文库,用于克隆的多功能DNA载体,靶基因的表达与分析,重组蛋白质高效表达和纯化试剂盒,细胞转染试剂和细胞培养工具,各种固体和溶解状态的酶,基因组学和蛋白组学研究产品(PCR,核酸,蛋白质纯化分析),各种抗体、细胞因子、生长因子、重组蛋白质,高级荧光试剂和试剂盒(蛋白质标记试剂盒,标记抗体,细胞染色试剂,钙指示器),亲和层析仪,凝胶分析工具——凝胶柱和凝胶电泳附件。
Bacillus megaterium Expression System
Bacillus subtilis Expression Vectors
pBacTag Tagging Vectors
pPICHOLI Shuttle Vector System
Broad-Host-Range Vectors pBBR122 & pBHR1
Fusion Protein Cloning System PheBo
Fusion Protein Cloning System pAX
BRP Plasmid and Competent BRP Cells
pPEPTIDE Cloning Vector
Promoter-Trap Vector pBBR RESO
pORF-CLONE Vector System
Exontrap Cloning Vector
PCR Cloning Vector p3T
Suicide Vector pCorrectClone
ssDNA-Production and Expression in pMEX
His-tag Cloning Vector pEG-His1
Multiple Cloning Site Vector pMCS5
Your partner for unique Two- and One-Hybrid-Systems, Phagemid Display, Genomic Libraries, DNA Vector Systems, Cell Biology and Antibodies, DNA and Protein Tools, Fluorescence Technology, Affinity Chromatography, and Lab Supplies
about MoBiTec
History
nnovative Tools for Molecular & Cell Biology
MoBiTec GmbH is a privately held company that was founded by Prof. Dr. Stephan Diekmann in 1987. The close relation to the Max-Planck Society as well as the location in Goettingen, one of Germany’s leading research centers, facilitated close cooperation with some of Germany’s most inventive scientists. In addition, MoBiTec has always been operating extensively with scientists all over Europe and North America, including those from the Pasteur Institute in France and Vrije University in the Netherlands. By commercializing the brilliant ideas of many researchers, MoBiTec is able to offer a unique range of high quality products with simplified protocols making lab life easier and leaving more time for actual research.
Products include e. g. two- & one hybrid systems for in vivo detection of protein-protein or DNA-protein interactions, a phagemid display system for screening specific peptide ligands and selecting novel proteins with specific binding properties, convenient Lambda PS genomic libraries; versatile DNA vectors for cloning, expressing and analyzing target genes, kits for efficient expression and purification of recombinant proteins, cell transfection reagents and cell culture tools, immobilized and soluble enzymes, many products for genomics and proteomics research (e. g. for PCR, nucleic acid & protein purification and analysis) , numerous antibodies, cytokines, growth factors and recombinant proteins, superior fluorescence reagents and kits (e. g. protein labeling kits, labeled antibodies, cell staining reagents, calcium indicators), affinity chromatography products, gel analysis tools as well as general laboratory equipment such as lab columns and gel electrophoresis accessories.
In parallel to its own product lines, MoBiTec distributes products from several international companies in Germany including fluorescent probes and research chemicals (Molecular Probes, AnaSpec, Teflabs), quantum dots (Evident Technologies), a variety of DNA purification kits and DNA polymerases (Edge Biosystems, TaKaRa), diagnostic tools (Biomerica, Cytocell), gel elecrophoresis accessories (Amresco), glycobiology products (Dextra Laboratories), high-quality biochip products (GenTel), cell transfection reagents (Mirus) as well as antibodies, proteins, substrates, fine chemicals and kits for molecular and cell biology - in particular virology, immunology, neurobiology, apoptosis, signal transduction, cell proliferation, cytotoxicity and cancer research (MBL, A.G. Scientific, TaKaRa, Amresco, ViroGen, AnaSpec).
MoBiTec offers a total service, beginning with the clear and full documentation of products extending to individual advice and technical service for customers. MoBiTec products are distributed worldwide - in Germany from our home office, in other countries by distributors. Moreover, MoBiTec is continuously looking for new products and marketable technologies for licensing and is therefore strongly interested in scientific collaborations in order to develop innovative product ideas.
药品标准物质由中国药品生物制品检定所负责标定。中国药品生物制品检定所可以组织有关的省、自治区、直辖市药品检验所、药品研究机构或者药品生产企业协作标定国家药品标准物质。
药品标准物质分类包括:标准品、对照品、对照试剂、对照制剂、对照药材、标准量具或耗材、工作标准品、精品。
如果你需要购买 药品对照品、标准品,可以到中国药品生物制品检定所订购,也可以查询中国标准物质网订购。
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NBS 是 Bio Basic Inc 的一部分,我们能够在英国提供全方位的 Bio Basic 定制测序服务。Bio Basic 是世界上最大的专业基因制造商之一。15 年来,他们作为一家无声的 OEM,为全世界的研究人员合成了数百万个碱基对的基因。
现在,我们很高兴能够直接以其自己的名义提供 Bio Basic 著名的基因合成服务。受益于其寡核苷酸库和 DNA 测序设施,我们以业内最实惠的价格提供基因合成。
Bio Basic 提供各种基因服务以满足任何实验室的需求。从大型项目到小型定制项目,我们可以帮助您选择能为您带来最大利益的服务。
我们的基因合成过程
1
寡核苷酸合成
具有相似解链温度 (Tm) 的多个长寡核苷酸(长度约 50-60 个碱基)根据所需的最终基因序列化学合成为片段。寡核苷酸被设计为通过重叠序列相互组装。
2
基因组装
使用 PCR 技术,寡核苷酸片段被组装成长达 1kb 的双链 DNA 块。然后使用 PCR 再次组装和扩增这些块,以创建一个大的双链 DNA 构建体。
3
克隆
合成的 DNA 被插入到特定的载体中。然后分离并扩增载体。除非另有说明,使用的默认载体是 pUC57-Amp 载体。
单击此处查看我们的矢量列表。
4
选择菌落
菌落生长,然后选择合适的含有目的合成基因的菌落。
5
DNA测序
所有最终基因都通过 DNA 测序验证,只有那些具有正确序列的基因才会被选中。这确保合成的基因符合项目开始时计划的规格。在这里,使用 Sanger 测序是因为它的读取长度长,运行时间短。
6
冻干
应用进一步的 QC 措施并将基因冻干到微量离心管中。该基因现已准备好用于下游应用。
芯片上的基因
通过引入下一代基因合成方法 - CHIP 技术,预计未来几年基因合成的价格将显着降低。因此,拥有一个能够适应这个不断变化的市场的合作伙伴将是一笔巨大的财富。
BioBasic 致力于通过对并行芯片基因合成技术等新技术的战略投资来保持市场竞争力。
拥有 BioBasic 作为您的合作伙伴将使您能够继续满足并超越您的期望和需求,而不会因在这个不断变化的市场中更换合作伙伴而带来的压力和并发症越来越大。
链接:http://www.nsjbio.com/
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NSJ BioreagentsX品牌 历史介绍:苏州蚂蚁淘生物科技有限公司,作为一家生命科学领域的高科技生物公司,目前代理销售二十多家欧美著名生物技术公司的产品,覆盖了免疫学、细胞生物学、分子生物学、药物筛选、生物制药、食品检测、疫苗生产等领域。
我问了两个实验员,一个说可以,一个说不行。
说不行的理由:1:会把RNA和DNA打断。2:超声破碎过程中会产热,会降解RNA。
请问有人用过破碎仪提取总RNA或基因组DNA吗?如果能,请问电压调到多少?还有哪些要求。
另外关于破碎仪消毒的问题,能用酒精棉球擦拭,再用无菌棉片檫干,再放入trizol中超声破碎吗?还有没有更好的方法?
谢谢!
LKT Labs
LKT LabsX品牌 历史介绍:苏州蚂蚁淘生物科技有限公司,作为一家生命科学领域的高科技生物公司,目前代理销售二十多家欧美著名生物技术公司的产品,覆盖了免疫学、细胞生物学、分子生物学、药物筛选、生物制药、食品检测、疫苗生产等领域。
苏州蚂蚁淘生物科技有限公司成立于2017年,是一家专业提供实验室整体采购方案的专业公司,为广大采购和实验室解决了大量问题,几年来已经成为所有合作伙伴最优秀的合作伙伴,我们一直秉承信誉第一的原则,坚持不卖假货,哪怕利润损失也要为客户提供最好的产品
我们已经和国外500多个品牌建立了贸易往来,并在国际上享有相当知名度,现已经成为国外近100多个品牌中国指定代理,并成为其中国最优秀的代理商