Thermo Reverse Transcriptase
- First-strand cDNA synthesis
- Active at 42~55℃. Optimum at 50℃.
- RNaseH negative
- Superior performance in RT-PCR. Up to 12 kb or more
- High processivity and sensitivity
Products
| Cat.No. | Product | Size |
|---|---|---|
| RT10K | HelixCript™ Thermo Reverse Transcriptase | 10,000 units |
| RT50K | HelixCript™ Thermo Reverse Transcriptase | 50,000 units |
| RT10KN | HelixCript™ Thermo Reverse Transcriptase (with dNTP Mix) | 10,000 units |
| RT50KN | HelixCript™ Thermo Reverse Transcriptase (with dNTP Mix) | 50,000 units |
- Description
- Data
- Details
- Documents
HelixCript™ Thermo Reverse Transcriptase, a Thermo stable and RNase H negative variant of M-MLV RTase, can synthesize cDNAs from purified RNA templates at a temperature range of 42℃ ~ 55℃ and especially shows the highest activity at 50℃. At the escalated temperature, the cDNA synthesis is enhanced partially due to the less internal structural formation of the template RNA and increased polymerization activity of RTase. The high processivity and productivity of HelixCript™ Thermo Reverse Transcriptase allows this enzyme to amplify the high yield of product and can synthesize cDNA of target gene up to 12 kb from RNA template. This enzyme is quite suitable to synthesize the first-strand cDNA for RT-PCR of target gene. The cDNA synthesis from total RNA or poly-(A)+ RNA is performed by random primer, oligo-d(T) primer, or gene-specific primer.
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如果用双抗体夹心法,第二个抗体可以用多抗,这样保证你的抗原会被结合,多抗可以直接带HRP标记或者可以用HRP标记二抗去和多抗结合。
(2)封闭:将已包被的板用洗涤液洗涤2次,每孔加入120 μL封闭液,封闭一段时间,取出,甩干备用。
(3)竞争反应:在制备好的板中每孔加入50 μL系列浓度的标准品溶液和50 μL稀释好的酶标抗体溶液,孵育一段时间。
(4)洗板:用洗涤液洗涤5次。
(5)显色反应:每孔加入显色溶液100 μL,孵育一段时间。
(6)终止:每孔各加入终止液,在酶标仪上测定各孔的吸光值。

