Thermo Reverse Transcriptase
- First-strand cDNA synthesis
- Active at 42~55℃. Optimum at 50℃.
- RNaseH negative
- Superior performance in RT-PCR. Up to 12 kb or more
- High processivity and sensitivity
Products
| Cat.No. | Product | Size |
|---|---|---|
| RT10K | HelixCript™ Thermo Reverse Transcriptase | 10,000 units |
| RT50K | HelixCript™ Thermo Reverse Transcriptase | 50,000 units |
| RT10KN | HelixCript™ Thermo Reverse Transcriptase (with dNTP Mix) | 10,000 units |
| RT50KN | HelixCript™ Thermo Reverse Transcriptase (with dNTP Mix) | 50,000 units |
- Description
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HelixCript™ Thermo Reverse Transcriptase, a Thermo stable and RNase H negative variant of M-MLV RTase, can synthesize cDNAs from purified RNA templates at a temperature range of 42℃ ~ 55℃ and especially shows the highest activity at 50℃. At the escalated temperature, the cDNA synthesis is enhanced partially due to the less internal structural formation of the template RNA and increased polymerization activity of RTase. The high processivity and productivity of HelixCript™ Thermo Reverse Transcriptase allows this enzyme to amplify the high yield of product and can synthesize cDNA of target gene up to 12 kb from RNA template. This enzyme is quite suitable to synthesize the first-strand cDNA for RT-PCR of target gene. The cDNA synthesis from total RNA or poly-(A)+ RNA is performed by random primer, oligo-d(T) primer, or gene-specific primer.
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的试剂:免疫吸附剂、结合物和酶的底物。
完整的ELISA试剂盒包含以下各组分:
(1)已包被抗原或抗体的固相载体(免疫吸附剂);
(2)酶标记的抗原或抗体(结合物);
(3)酶的底物;
(4)阴性对照品和阳性对照品(定性测定中),参考标准品和控制血清(定量测定
中);
(5)结合物及标本的稀释液;
(6)洗涤液;
(7)酶反应终止液。
在这种测定方法中有3种必要的试剂:①固相的抗原或抗体(免疫吸附剂) ②酶标记的抗原或抗体(标记物)③酶作用的底物(显色剂)
测量时,抗原(抗体)先结合在固相载体上,但仍保留其免疫活性,然后加一种抗体(抗原)与酶结合成的偶联物(标记物),此偶联物仍保留其原免疫活性与酶活性,当偶联物与固相载体上的抗原(抗体)反应结合后,再加上酶的相应底物,即起催化水解或氧化还原反应而呈颜色。
其所生成的颜色深浅与欲测的抗原(抗体)含量成正比。 这种有色产物可用肉眼、光学显微镜、电子显微镜观察,也可以用分光光度计(酶标仪)加以测定。其方法简单,方便迅速,特异性强。向左转|向右转

