Recombinant Human His6-SUMO1 Protein, CF Summary
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
UL-715
| Formulation | X mg/ml (X μM) in 50 mM HEPES pH 8.0, 150 mM NaCl and 1 mM DTT |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: SUMO1
Human Small Ubiquitin-like Modifier 1 (SUMO1), also known as Sentrin, UBL1, and SMT3C, is synthesized as a 101 amino acid (aa) propeptide with a predicted molecular weight of 11.5 kDa. Human SUMO1 is the most unique of the four identified SUMO proteins and shares only 44%, 47%, and 41% aa sequence identity with SUMO2, SUMO3, and SUMO4, respectively. In contrast, human SUMO1 shares 100% aa sequence identity with the mouse ortholog. SUMOs are a family of small, related proteins that can be enzymatically attached to a target protein by a post-translational modification process termed SUMOylation (1-3). All SUMO proteins share a conserved Ubiquitin domain and a C-terminal diglycine cleavage/attachment site. Following cleavage of a four aa C-terminal prosegment, the C-terminal glycine residue of SUMO1 is enzymatically attached to a lysine residue on a target protein. In humans, SUMO1 is conjugated to a variety of molecules in the presence of the SAE1/UBA2 SUMO-activating (E1) enzyme and the UBE2I/Ubc9 SUMO-conjugating (E2) enzyme (4,5). In yeast, the SUMO-activating (E1) enzyme is Aos1/Uba2p (6). SUMOylation can occur without the requirement of a specific SUMO ligase (E3), where SUMO1 is transferred directly from UBE2I/Ubc9 to specific substrates. In Alzheimer"s disease models SUMO1 has been shown to influence the generation of Amyloid-beta peptide by promoting the accumulation of BACE-1 (7). Covalent modification of Phosphatase and Tensin Homolog Deleted on Chromosome (PTEN) by SUMO1 is thought to regulate tumorigenesis by retaining PTEN at the plasma membrane, an effect that suppresses PI 3-Kinase/Akt-dependent tumor growth (8).
The ubiquitin-like SUMO-1 is conjugated to a variety of proteins in the presence of UbcH9 and the SAE1/SAE2 (human) or Aos1/Uba2 (yeast) activating enzyme. SUMO-1 is derived from the precursor pro-SUMO-1 (Accession # NM_003352). Human SUMO-1 shares 46% and 47% identity with SUMO-2 and SUMO-3 respectively. SUMOylation can occur without the requirement of a specific E3 ligase activity, where SUMO is transferred directly from UbcH9 to specific substrates. SUMOylated substrates are primarily localized to the nucleus (RanGAP-1, RANBP2, PML, p53, Sp100, HIPK2), but there are also cytosolic substrates (I kappa B alpha, GLUT1, GLUT4). SUMO modification has been implicated in functions such as nuclear transport, chromosome segregation, and transcriptional regulation.
- Desterro, J.M. et al. (1997) FEBS. Lett. 417:297.
- Bettermann, K. et al. (2012) Cancer Lett. 316:113.
- Praefcke, G.J. et al. (2012) Trends Biochem. Sci. 37:23.
- Okuma, T. et al. (1999) Biochem. Biophys. Res. Commun. 254:693.
- Tatham, M.H. et al. (2001) J. Biol. Chem. 276:35368.
- Johnson, E.S. et al. (1997) EMBO J. 16:5509.
- Yun, S.M. et al. (2012) Neurobiol Aging. [Epub ahead of print].
- Huang, J. et al. (2012) Nat. Commun. 3:911.
Citations for Recombinant Human His6-SUMO1 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products.The data collected includes not only links to publications in PubMed,but also provides information about sample types, species, and experimental conditions.
2Citations: Showing 1 - 2Filter your results:
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- Functional Insights into ANP32A-Dependent Influenza A Virus Polymerase Host RestrictionAuthors: P Domingues, BG HaleCell Rep, 2017;20(11):2538-2546.Species: HumanSample Types: ProteinApplications: Bioassay
- Functional Insights into ANP32A-Dependent Influenza A Virus Polymerase Host RestrictionAuthors: P Domingues, BG HaleCell Rep, 2017;20(11):2538-2546.Species: HumanSample Types: Cell LysatesApplications: Bioassay
FAQs
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过表达慢病毒
＞10^8 PFU/ml
干扰慢病毒
＞10^8 PFU/ml
如果用组织DNA提取试剂盒，提出来的就是组织细胞的DNA了
从1～2个T75培养皿的感染细胞培养液中纯化腺病毒
产品简介
本系列试剂盒可快速，高效地从腺病毒感染的细胞培养液中分离纯化腺病毒，相比于传统CsCl超速离心的腺病毒病毒纯化方式（需要24个小时才能完成纯化），本试剂盒可以在1个小时内完成病毒纯化，操作简单，快速，纯化率高，纯化到的病毒颗粒可直接用于下游实验，如细胞和动物感染。
产品特点
?操作简单，快速，可以在1个小时内完成病毒纯化，不依赖于超速离心操作。
?纯化效率高：小量纯化，可从1~2个T75瓶培养的细胞培养液中纯化病毒颗粒高达1x1012VPs
?每个纯化柱，可以重复利用一次，用于纯化相同种类的腺病毒。
保存条件
纯化柱和脱盐柱保存于4℃，其它组分室温保存。
试剂盒组分
Catalog#
V1160-01
Notes
Preps
10
MiniColumns
5
Canbeusedtwice
(Storeat4°C)
Press-OnCap
5
StoreatRT
DesaltingTube*
1
Canberegenarated
(Storeat4°C)
15mLCollectionTube
10
StoreatRT
10xWashBuffer
30mL
StoreatRT
2xElutionBuffer
30mL
StoreatRT
RegenerationBuffer
30mL
StoreatRT
联系方式：
Biomiga(中国）
电话：0573-82651206
QQ:441931287
联系人：张小姐
如果PCR产物不是很纯，或者PCR扩增条带比较小，PCR产物前面又有较多引物二聚体时，用胶回收，其余用PCR产物纯化试剂盒。
pcr纯化试剂盒和胶回收试剂盒的区别：
PCR纯化试剂盒：是直接水溶解的PAC产物就可以回收，回收效率高，但是只适合单一条带需要纯化测序的时候使用。
PCR凝胶试剂盒：是在PCR产物是混合物，有多条杂带的情况下，先跑胶将杂带分离，然后在将所要的条带位置的胶切下回收，后者的回收效率低，但是很纯净。
胶回收试剂盒操作步骤：
配制琼脂糖EB凝胶,电泳以分离DNA片段。任何类型或等级的琼脂糖都可以使用。
电泳足够时间后，在紫外灯下小心地把所需的DNA的片段切下来。并尽量去除多余的凝胶。
称取空离心管的重量，切下带目的片段的凝胶装在1.5ml离心管中并称其重量，求出凝胶块的重量，近似地确定其体积。一般情况下，凝胶的密度为1g/ml，于是凝胶的体积与重量的关系可按下面换算：凝胶薄片的重量为0.2g 则其体积为0.2ml；加入等倍凝胶体积的Binding Buffer，把混合物置于55℃~65℃水浴中温浴7min至凝胶完全融化，其间每隔2-3分钟混匀一次；
转移700μl的DNA-琼脂糖溶液到一个HiBindTM DNA柱子，并把柱子装在一个干净的2ml收集管内，室温下，10,000×g离心1min，弃去液体。
将柱子重新套回收集管中，加300μl Binding Buffer至HiBind DNA 柱子中；室温下，10,000×g离心 1分钟，去弃滤出液；这一步相当关键，不要忽略此步。
将柱子重新套回收集管中，加入700μl SPW Wash buffer至HiBind DNA柱子中，室温下，10,000×g离心1分钟，去弃滤出液；注：SPW Wash buffer在使用前必须按瓶子标鉴要求用无水乙醇进行稀释。
将柱子重新套回收集管中，重复加入700μl SPW Wash buffer至HiBind DNA柱子中，室温下，10,000×g离心1分钟，去弃滤出液；
弃去液体，将空柱子重新套回收集管中，10,000×g离心1min以甩干柱基质残余的液体。
这步可以去除柱子基质上残余的乙醇，不要省略此步―――对得到好的DNA产量是十分重要的。
把柱子装在一个干净的1.5ml离心管上，加入30~50μl洗脱液或灭菌水上柱子膜上，10,000×g离心1分钟，离心管中的溶液就是纯化的DNA产物，保存于-20度。
试剂里面含有的是病毒的抗原，现在基本都是人工合成或提取的，跟病毒完全是两回事
