| Isotype | IgG |
| Product Type | Polyclonal Antibody |
| Units | 250 µg |
| Host | Sheep |
| Species reactivity | MouseRat |
| Application | Immunoprecipitation |
Backgroundp38HOG kinase isactivated in responce to numerous extracellular signalling events.Stress stimuli such as UV irradiation, thermal stress, inflammatory cytokines and bacterial products such as LPS stimulate the activation of p38HOG kinase. p38HOG kinase is necessary for the cellular inflammatroy responce in animals.
Synonyms: sheep anti p38HOG kinase, p38 HOG kinase
Source
Immunogen: Full length p38 HOG kinase protein
Product
Product Form: Unconjugated
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide
Purification Method: Ammonium Sulfate Precipitation
Concentration: See vial for concentration
ApplicationsApplications: 1-10 ug/ml for immunoprecipitation of the catalyticaly active form ofp38HOG kinase in vitro kinase assay.
Functional Analysis: Immunoprecipitation
StorageProduct should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles
Product Stability: Products are stable for one year from purchase when stored properly
Shipping Conditions: Room Temperature
CautionThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Exalpha Biologicals accepts no liability for any inaccuracies or omissions in this information.
Safety Datasheet(s) for this product:EA_Sodium Azide/wp-content/uploads/SDS/Antibody SDS with Sodium AzideV2.pdf
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ELISA双抗体夹心法(enzyme linked immunosorbent assay——sandwich technique)的原理是将特异性抗体结合到固相载体上形成固相抗体,然后和待检血清中的相应抗原结合形成免疫复合物,洗涤后再加酶标记抗体,与免疫复合物中抗原结合形成酶标抗体-抗原-固相抗体复合物,加底物显色,判断抗原含量。
生物帮有相关介绍。编码RNA http://doc.bio1000.com/show-3399.html
请大家帮帮忙,第一步加完抗原后必须封闭吗
表面抗体是双抗原夹心法
E抗体和核心抗体是竞争抑制法或间接法
在这种测定方法中有3种必要的试剂:①固相的抗原或抗体(免疫吸附剂) ②酶标记的抗原或抗体(标记物)③酶作用的底物(显色剂)
测量时,抗原(抗体)先结合在固相载体上,但仍保留其免疫活性,然后加一种抗体(抗原)与酶结合成的偶联物(标记物),此偶联物仍保留其原免疫活性与酶活性,当偶联物与固相载体上的抗原(抗体)反应结合后,再加上酶的相应底物,即起催化水解或氧化还原反应而呈颜色。
其所生成的颜色深浅与欲测的抗原(抗体)含量成正比。 这种有色产物可用肉眼、光学显微镜、电子显微镜观察,也可以用分光光度计(酶标仪)加以测定。其方法简单,方便迅速,特异性强。向左转|向右转
2.加大辣根过氧化物酶标记的链霉亲和素;
1.标准曲线的标准品是否一定要梯度稀释,为什么?我试过非梯度稀释的,也可以达到线性R2=0.99.
2.我用了CurveExpert做标曲,自动搜索后发现有10种提供的方程,各种形式的,其中一个十分适合我的实验结果(LogisticModel),而其他的感觉又不适合,因为结果常常为负值。这又是为啥捏?
3.实验的酶标仪最大OD值可以测到4,如果我的测量结果在1.3,是否像其他人所说的>1了就不准确了。
4.利用夹心法进行定量分析是否一定要使用线性方程?
不好意思啊,一下问了这么多问题,最近做了一个月的ELISA,完全摸不清头脑啊。谢谢各位了

