ProductType | ControlLysate |
Units | 100µl |
Application | WesternBlotWesternBlotting |
BackgroundEDG-1belongstoafamilyofG-proteincoupledreceptorswhoseligandsarelysophospholipids.TheligandforEDG-1issphingosine-1-phosphate.Thereare8knownmembersoftheEDGreceptorfamilyandtheyareimplicatedinmediatinggrowthrelatedeffectssuchasinductionofcellularproliferation,alterationsindifferentiationandsurvivalandsuppressionofapoptosis.Theyalsoevokecellulareffectorfunctionsthataredependentoncytoskeletalresponsessuchascontraction,secretion,adhesionandchemotaxis.EDGreceptorsaredevelopmentallyregulatedanddifferintissuedistribution.TheycoupletomultipletypesofGproteinstosignalthroughrasandMAPkinase,rho,phospholipaseCandseveralproteintyrosinekinases.EDG-1isexpressedincardiovascular,leukocyte-containingandothertissues.
Synonyms:EDG1(S1P1)TransfectedCellPositiveControlLysate
Product
ProductForm:Unconjugated
Formulation:Providedin10%glycerol,0.063MTris-HCl(pH6.8),2%SDSand0.002%bromophenolblue,5%2-mercaptoethanol
Concentration:Seevialforconcentration
ApplicationsForuseasapositivecontrolwithExalpha"sEDG1(S1P1)polyclonalantibody(Cat.No.X1093P)forWesternblotanalysis.
FunctionalAnalysis:WesternBlotting
StorageProductshouldbestoredat-20ºC.Aliquottoavoidfreeze/thawcycles
ProductStABIlity:Productsarestableforoneyearfrompurchasewhenstoredproperly
ShippingConditions:Shipatambienttemperature,freezeuponarrival
CautionThisproductisintendedFORRESEARCHUSEONLY,andFORTESTSINVITRO,notforuseindiagnosticortherapeuticproceduresinvolvinghumansoranimals.Itmaycontainhazardousingredients. PleaserefertotheSafetyDataSheets(SDS)foradditionalinformationandproperhandlingprocedures.Disposeproductremaindersaccordingtolocalregulations.Thisdatasheetisasaccurateasreasonablyachievable,butExalphaBIOLOGicalsacceptsnoliabilityforanyinaccuraciesoromissionsinthisinformation.
SafetyDatasheet(s)forthisproduct:EA_Glycerol/wp-content/uploads/SDS/AntibodySDSwithglycerolV2.pdf
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1.标准曲线的标准品是否一定要梯度稀释,为什么?我试过非梯度稀释的,也可以达到线性R2=0.99.
2.我用了CurveExpert做标曲,自动搜索后发现有10种提供的方程,各种形式的,其中一个十分适合我的实验结果(LogisticModel),而其他的感觉又不适合,因为结果常常为负值。这又是为啥捏?
3.实验的酶标仪最大OD值可以测到4,如果我的测量结果在1.3,是否像其他人所说的>1了就不准确了。
4.利用夹心法进行定量分析是否一定要使用线性方程?
不好意思啊,一下问了这么多问题,最近做了一个月的ELISA,完全摸不清头脑啊。谢谢各位了
我现在用商品化的试剂盒进行夹心法ELISA测定某抗体的浓度,盒子给定了4个标准品,标准品抗体浓度为70000~1000单位,测定步骤中要求对样品进行1:1000稀释后测定。
但是有的样本按照1:1000稀释后,最终OD值大于70000浓度的标准品的OD值,然后使用ELISACALC软件进行四参数拟合,超过了标准曲线的范围,就算不出来这个样品的浓度,但是如果在1000-70000之间的OD值就可以算出相应浓度。请教大神们,接下来我是否可以对样品进行1:2000,1:4000浓度稀释,算出结果后再×2、×4,算作此样品的浓度呢?还是直接给一个>70000的定性结果?或者可以有能算出来的其他软件?
Ps试剂盒中提及Dilutionlinearity(稀释线性?)为141%,这个是什么意思呢?
多谢!!
1. 直接法测定抗原 2.间接法测定抗体 3.双抗体夹心法测定抗原4. 竞争法测定抗原 厚百生物专业提供各类生化实验试剂、仪器、耗材,提供技术服务,满足您的实验室常规采购需求。厚百,让您更省心!
请大家帮帮忙,第一步加完抗原后必须封闭吗