
Catalog number | TE187S-100 |
Product type | ELISA |
Quantity | 96 Tests (12x8 breakable strip wells) |
Standard range | 0.1-18 ng/ml |
Analytical Sensitivity | 0.1 ng/ml |
Sample volume | 25 µl/well |
Species | Mouse or Rat |
Application | The Calbiotech Mouse/Rat Testosterone ELISA is for the quantitative determination of Testosterone concentration in mouse/rat serum or plasma. |
Principle | The Calbiotech Mouse/Rat Testosterone ELISA is based on the principle of competitive binding between Testosterone in the test specimen and Testosterone-HRP conjugate for a constant amount of rabbit anti-Testosterone. In the incubation, goat anti-rabbit IgG-coated wells are incubated with 25ml of Testosterone standards, controls, patient samples, 100 ml Testosterone-HRP conjugate reagent and 50ml rabbit anti-Testosterone reagent at room temperature 60 minutes. During the incubation, a fixed amount of HRP-labeled Testosterone competes with the endogenous Testosterone in the standard, sample, or quality control serum for a fixed number of binding sites of the specific Testosterone antibody. Thus, the amount of Testosterone peroxidase conjugate immunologically bound to the well progressively decreases as the concentration of Testosterone in the specimen increases. Unbound Testosterone peroxidase conjugate is then removed and the wells washed. Next, a solution of TMB Reagent is then added and incubated at room temperature for 15 minutes, resulting in the development of blue color. The color development is stopped with the addition of stop solution, and the absorbance is measured spectrophotometrically at 450nm. |
Storage and Stability | Product should be stored at 2-8 °C. Product is stable for 24 months from the date of manufacturing. |
Precautions | For research use only. Not for use in diagnostic procedures. |
References | 1. Chen, A., Bookstein, J.J., Meldrum, D.R., Diagnosis of a testosterone-secreting adrenal adenoma by selective venous catheterization. Fertil. Steril., 1991; 55: 1202-1203. 2. Granoff, A.B. and Abraham, G.E., Peripheral and adrenal venous levels of steroids in a patient with virilizing adrenal adenoma. Obstet. Gynecol., 1979; 53:111-115. 3. Bricaire, C., Raynaud, A., Benotmane, A., et al., Selective venous catheterization in the evaluation of hyperandrogenism. J. Endocrinol Invest., 1991; 14: 949-956. 4. Heinonen, P.K., Androgen production by epithelial ovarian tumours in post-menopausal women. Maturitas, 1991; 13: 117-117-122 5. Tietz, N.W. ed., Clinical Guide to Laboratory Tests, 3rd Edition, W.B. Saunders, Co., Philadelphia, 1995: 578-580. 6. USA Center for Disease Control/National Institute of Health Manual, “Biosafety in Microbiological and Biomedical Laboratories"”84 7. ICN Guide to Endocrine Testing. Diagnostic Division, ICN Biomedicals, Inc. pp. 2:33-35; 3:4-6. |
Mouse/Rat Estradiol ELISA

Mouse/Rat Cotinine ELISA

Mouse/Rat HSV-1 IgG ELISA

Mouse/Rat Prolactin ELISA

Mouse/Rat T3, Total ELISA

Mouse/Rat T4, Total ELISA

Mouse/Rat ACTH Ultra Sensitive lumELISA
ebiomall.com






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从1~2个T75培养皿的感染细胞培养液中纯化腺病毒
产品简介
本系列试剂盒可快速,高效地从腺病毒感染的细胞培养液中分离纯化腺病毒,相比于传统CsCl超速离心的腺病毒病毒纯化方式(需要24个小时才能完成纯化),本试剂盒可以在1个小时内完成病毒纯化,操作简单,快速,纯化率高,纯化到的病毒颗粒可直接用于下游实验,如细胞和动物感染。
产品特点
?操作简单,快速,可以在1个小时内完成病毒纯化,不依赖于超速离心操作。
?纯化效率高:小量纯化,可从1~2个T75瓶培养的细胞培养液中纯化病毒颗粒高达1x1012VPs
?每个纯化柱,可以重复利用一次,用于纯化相同种类的腺病毒。
保存条件
纯化柱和脱盐柱保存于4℃,其它组分室温保存。
试剂盒组分
Catalog#
V1160-01
Notes
Preps
10
MiniColumns
5
Canbeusedtwice
(Storeat4°C)
Press-OnCap
5
StoreatRT
DesaltingTube*
1
Canberegenarated
(Storeat4°C)
15mLCollectionTube
10
StoreatRT
10xWashBuffer
30mL
StoreatRT
2xElutionBuffer
30mL
StoreatRT
RegenerationBuffer
30mL
StoreatRT
联系方式:
Biomiga(中国)
电话:0573-82651206
QQ:441931287
联系人:张小姐
病毒液通过pre-filterdisc的时候是不是要制造一个真空环境产生压力差使液体通过?这个时候是用自备的针管还是试剂盒配的5毫升的针管啊?
试剂里面含有的是病毒的抗原,现在基本都是人工合成或提取的,跟病毒完全是两回事
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