The TALON PMAC Magnetic Phospho Enrichment Kit combines the phosphospecificity of our TALON-based Phosphoprotein Enrichment Kit with the convenience of magnetic bead separation to provide a simple, rapid, metal affinity-based method for isolating microgram quantities of phosphorylated proteins from mammalian cells and tissues. Magnetic particles in the beads facilitate quick and easy purification of microscale quantities of phosphoproteins when placed on a magnetic separator...
The TALON PMAC Magnetic Phospho Enrichment Kit combines the phosphospecificity of our TALON-based Phosphoprotein Enrichment Kit with the convenience of magnetic bead separation to provide a simple, rapid, metal affinity-based method for isolating microgram quantities of phosphorylated proteins from mammalian cells and tissues. Magnetic particles in the beads facilitate quick and easy purification of microscale quantities of phosphoproteins when placed on a magnetic separator.
Isolate cytosolic and membrane-bound proteins
Each kit supplies a complete set of buffers along with our Phospho Magnetic Beads for group-specific enrichment of all types of phosphoproteins, both cytosolic and membrane-bound, that contain a phosphorylated amino acid side chain—including serine, tyrosine, or threonine.
The extraction/loading buffer contains a non-ionic detergent to extract cellular proteins. Wash and elution buffers are detergent-free. If desired, phosphoproteins can be eluted in pH 9.0 buffer, which is more suitable for downstream applications.
Microscale purification in small elution volumes
Phospho Magnetic Beads are supplied as a 5% suspension, with a demonstrated binding capacity of 400 µg of alpha-casein per ml of suspension. Different amounts of beads may be used, depending on the initial sample concentration. Phosphoproteins can be eluted in small volumes (50–200 µl) to yield concentrated samples.
Highly selective enrichment of phosphoproteins
Only a small percentage of all cellular proteins are phosphorylated, so it is often necessary to enrich for this fraction before beginning an analysis. The TALON PMAC Magnetic Phospho Enrichment Kit can be used with any mammalian cell type or tissue sample. The yield of phosphorylated protein varies with different cell lines. The enrichment procedure is highly efficient.
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请具体点，把联系方式和价位都说一下好吗？
我也想问一下。
求采纳
Clontech的非常好, 感觉. 效果不好可能是RNA问题, 或RTase的问题. 因为目前clontech的RTase是Takara的产品，而非原来clontech的产品，可单购。
请采纳。。。。
做一次5-RACE的话，克隆10个以上基因应该是可以的。本数据来源于百度地图，最终结果以百度地图最新数据为准。
