分析测试百科网 WIKI资讯 AA&MetaboliteQuantitationofCellPelletsPostAALabeling Extraction:1) Following spin, save supernatant for analysis. Be extremely careful not to disturb the pellet since it is somewhat dispersed.2) Immediately upon removing supernatant from each tube, add 3 mls of chloroform-methanol, 1:2, and vortex vigorously!!-- It is necessary to add the C:M immediately in order to get good dispersion of the pellet. If too much time passes prior to resuspension, the pell......阅读全文 CellTrace™ CFSE Cell Proliferation Kit 实验概要The CellTrace™ CFSE Cell Proliferation Kit provides a versatile and well-retained cell-tracing reagent in a convenient and easy-to-use 重组DNA的分离、克隆与测序实验手册-7 III. Methods for DNA isolationA. Large scale double-stranded DNA isolationThe method used for the isolation of large scale cosmid and plasmid DNA is a Biosynthetic labeling How long should cells be labeled?The ideal length of time to label cells depends on the protein of interest and the label that you are using. If RNA FISH on cultured cells in interphase IntroductionFluorescencein situhybridization (FISH) has become a widely used method in genome and molecular genetic studies. The technique 细胞组分和细胞器——细胞骨架 Fixation and Immunofluorescence of the Cytoskeleton(Mitchison Lab)Recycling Tubulin(Mitchison Lab)Labeling Tubulin Basic Method for Indirect Immunofluorescence Labeling Basic Method forIndirectImmunofluorescence LabelingBackgroundThis is the method forindirectimmunofluorescence labeling; that i 重组DNA的分离、克隆与测序实验手册-6 Electroporation ProtocolPreparation of Electro-competent Cells:1. Grow XL1-Blue cells on a tetracycline plate (20 ug tet/ml of LB agar)2. Inoculate 3 体外荧光法检测核内体早期动力学 A fluorescence-basedin vitroassay for investigating early endosome dynamicsSina V Barysch1,2, Reinhard Jahn1 Silvio O Rizzoli2A Immunohistochemistr... 实验概要Immunohistochemistry is a classic technique used for the localization of antigenic target molecules in ­tissue. The method exploits the princi New Brunswick S41i CO2 恒温摇床进行间充质干细胞扩增（一） A Novel Method for the Expansion of Mesenchymal Stem Cells using an Eppendorf New Brunswick™ S41i CO2 Incubator ShakerKhandaker Siddiquee and Ma Sha, SBA-5型CO2气体分析仪参与作物对大气变化响应的相关研究2 2011CJ Bernacchi, ADB Leakey, BA Kimball, DR Ort (2011) Growth of soybean at future tropospheric ozone concentrations decreases canopy evapotransp ChIP-Chip E. coli AbstractChIP-Chip stands forChromatinImmunoprecipitation and chip in the sense of DNA microarray. It is a technique to determine the genom 瑞士苏黎世联邦理工学院的研究人员领导的一个研究小组，已制定了一个质谱工作流，用于对整个蛋白质组进行高通量的绝对定量。 据ETH的研究者、该研究的领头人Ruedi Aebersold说，对于多种形态，该技术实现了目标蛋白质组的重现性定量，提供用于 Simultaneous analysis of DNA content Simultaneous analysis of DNA content and surface immunophenotype using gentle ethanol fixation techniques.William Telford. Louis E. King a Fibroblast Cell Systems-1 THESE INSTRUCTIONS APPLY TO ORDERS CONTAINING THE FOLLOWING CELL PRODUCTSCryopreserved Cells(Single donor)CC-2511NHDF -Ad3 500,000 cells/cryovia 2010全国质谱大会大会报告（三） 诺华（Novartis）制药 Wang, Karen（王瑛琪）教授 来自诺华（Novartis）制药的Wang, Karen（王瑛琪）教授，做了题为\"Application of Mass Spectrometry in Pharmaceutical Drug Discovery Research Protocol for Dual Pulse Labeling Using EdU and BrdU Incorporation 实验概要The measurement of cell proliferation is fundamental to the assessment of cell health, genotoxicity, and drug efficacy. Proliferation Combined Flow Cytometric Measurement of Two Cell-Surface Antigens-2 DNA and RNA Staining6. Stain cells with 7-AAD:i. Resuspend the cells from Step5 in 0.5 mL of NASS containing10 µg/mL of 7-AAD. Incub Hematopoietic Stem Cell Targeting with Surface-1 Hematopoietic Stem Cell Targeting with Surface-Engineered Lentiviral VectorsEls VerhoeyenandFrançois-Loïc CossetAdapted fromGene Tra Genomic DNA Extraction - PureLink™ 实验概要The PureLink™ Genomic DNA Purification Kit allows rapid and efficient purification of genomic DNA. The kit is designed to efficiently Preparation of tubulin-2 DAY 2: Cycling preparation of MT protein.Keep the brains in an evacuated plastic ziplock bag buried in ice from the time of slaughter during transport 线粒体荧光探针大全：TMRM，Mitotracker，JC-1（5） Monoclonal Antibodies Specific for Proteins in the Oxidative Phosphorylation SystemOxidative phosphorylation (OxPhos) activity occurs in the mitochond 张祺屿等 过氧化物酶建立标记阐明神经突触连接模式由于突触连接的构成部分非常小（约数十纳米），通常借助电子显微镜来阐释神经元之间的突触连接模式（synaptic connectivity）。现有的大规模神经系统电镜重建数据（large-scale EM reconstruction of the nervous system）虽然可以用于构建无 An Integrative Procedure for Apoptosis Identification and Measurement IntroductionApoptosis is a normal physiological phenomenon put forward by Kerr [1]. It plays an important role in embryonic development, maintenance o 研究HIV治疗过程的单细胞免疫反应 Mario Roederer博士的团队通过研究单细胞来更好地描述在HIV治疗过程中免疫系统的特征。该团队使用非人类灵长目模式动物，用单细胞基因表达分析提高T细胞的鉴定。他们使用了先进的技术，包括18色 FACS系统和Fluidigm BioMark™ 系统进行单细胞基因表达分析。基本研究内容包括： 酵母染色体沉淀分析方法 ABSTRACTThis protocol describes a method for the detection of proteins bound to specific regions of chromatin in yeast. There are many variations of t FIXATION and DNA Staining for Cell Cycle Analysis BackgroundThis method of DNA staining utilizes ethanol to fix the cells and permeabilize the membrane, which allows the dye (Propidium Iodide) to ente Detection of apoptotic process in situ using immunocytochemical-2 B) TUNEL in situ procedureB.2.1 Materialsproteinase K (pK) (A2), H2O2, TdT buffer (A1), TdT enzyme (A2), biotinylated dUTP (A2), TB buffer (A1), Immunity同期刊登中国学者两项成果 来自清华大学医学院，以及中科院生物物理研究所等处的研究人员发表题为\"The Adaptor SAP Controls NK Cell Activation by Regulating the Enzymes Vav-1 and SHIP-1 and by Enhan Multicolour 3D-FISH in vertebrate cells-2 Small DNA-probes from cosmids or plasmids clonesThese kind of probes, especially plasmids, have become out of fashion for 3D-FISH due to their delicat