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Bpsbioscience/DCAMKL1, GST-tag/40047/10 µg
2025-05-12
Species: Human Host Species: Sf9 insect cells MW: 110 kDa Genbank #: NM_004734 Tag(s): N-terminal GST-tag a.a: full length Background:DCAMKL1 or doublecortin-like kinase 1 contains two N-terminal doublecortin domains (which bind microtubules and regulate microtubule polymerization), a C-terminal serine/threonine protein kinase domain (which shows substantial homology to Ca2+/calmodulin-dependent protein kinase), and a serine/proline-rich domain in between the doublecortin and the protein kinase domains (which mediates multiple protein-protein interactions) (1). DCAMKL1 is a microtubule-associated kinase that can undergo autophosphorylation. DCAMKL1 has microtubule-polymerizing activity that is independent of its protein kinase activity (2). DCAMKL1 is involved in several different cellular processes, including neuronal migration, retrograde transport, neuronal apoptosis and neurogenesis. Description:Recombinant humanDCAMKL1, also known as DCLK1,DCDC3A, DCLK, or KIAA0369, GenBankAccession No. NM_004734, full-lengthwith an N-terminal GST tag andexpressed in a baculovirus-infected Sf9cell expression system. Synonym(s): DCLK1; DCDC3A; DCLK; KIAA0369 Purity: ≥75% Specific Activity: 3.4 pmol/min/µg Assay Conditions:
Varying amounts of enzyme were incubated in 5 mM MOPS, pH 7.2, 2.5 mM β-glycero-phosphate, 4 mM MgCl2, 2.5 mM MnCl2, 1 mM EGTA, 0.4 mM EDTA, 0.01 mM DTT, 0.05 mM ATP, 5 μg of autocamtide 2 synthetic peptide substrate, and 4 μCi of [33P]-ATP was incubated at 30°C for 15 minutes. Then spot reaction on phosphocellulose paper, fix in 1% phosphoric acid, and assay with a scintillation counter.
Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol. Format: Aqueous buffer solution Storage / Stability:>6 months at -80°C.
Application(s): Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Reference(s): 1. Ohmae, S. et.al: Molecular identification and characterization of a family of kinases with homology to Ca(2+)/calmodulin-dependent protein kinases I/IV. J. Biol. Chem. 281: 20427-20439, 2006.2.Lin, P. T. et,al: DCAMKL1 encodes a protein kinase with homology to doublecortin that regulates microtubule polymerization. J. Neurosci. 20: 9152-9161, 2000. Warning(s): Avoid freeze/thaw cycles Scientific Category: Kinase/Serine-Threonine Product Type: Recombinant Protein Data shown is lot-specific. Contact us for specific information on other lots.本文链接： https://www.ebiomall.cn/b166-bps/info-1514604841.html
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