Bpsbioscience/ONE-Step™ Luciferase Assay System - 10 ml/60690-1/10 ml
Luciferase is the general term given to a class of oxidative enzymes that catalyze reactions that give off light, a process known as bioluminescence. In biology, researchers can take advantage of this reaction and use it as a readout for various biological processes. This has perhaps been exploited most in luciferase reporter cell lines where a promoter region from a gene of interest is placed immediately upstream of the coding sequence for luciferase. In this system, transcriptional activation of the gene of interest leads to a level of luciferase expression that is proportional to the level of gene activation.
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The level of activation is then assessed by lysing the cells and adding the luciferase substrate, D-luciferin. Using the One-Step Luciferase Assay System from BPS Bioscience, this is accomplished in a single step as the lysis buffer has been formulated with D-luciferin. After a brief incubation period, bioluminescence is read on a luminometer.
Description:The One-Step Luciferase Assay System is designed to be used for high-throughput, sensitive quantitation of firefly luciferase activity in mammalian cell culture. The reagent consists of two components, a Luciferase Reagent Buffer (Component A) and Luciferase Reagent Substrate (Component B). Component A and Component B are combined to form a working solution that contains all the necessary components for cell lysis and luciferase quantitation. This assay system has several features: • Sensitive – highly sensitive detection of firefly luciferase activity. • Stable – the signal output is stable for more than two hours, providing flexibility with regard to incubation time • Convenient – simple one-step, homogeneous protocol. • High-throughput – one-step homogeneous protocol minimizes handling steps to support high-throughput screening applications • Compatibility – works well with a variety of common media containing 0-10% serum and phenol red. • Instrumentation – does not require a luminometer with injectors.
Synonym(s): Luciferin, Firefly luciferase, luciferase buffer Format:COMPONENTS: ![](\"http://bpsbioscience.com/media/wysiwyg/format.JPG\")
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