Product Description:

IL-16 is a CD8+ T cell-derived cytokine that induces chemotaxis of CD4+ T cells, CD4+ monocytes, and eosinophils. Analysis by gel filtration suggests that, under physiological conditions, hIL-16 exists predominantly as a noncovalently linked multimer, but that some IL-16 may exist as a monomer. However, only the multimeric form appears to possess chemotactic activity, suggesting that receptor cross-linking may be required for activity. IL-16 also induces expression of IL-2 receptor (IL-2R) and MHC class II molecules on CD4 + T cells. Human and murine IL-16 show significant cross-species reactivity.

Technical Specifications:

• Species: Human
• Expression: E. coli Cell Expressed
• Purity: ≥98%
• Endotoxin: <1.0 EU/μg
• Molecular Mass: 12.4 kDa
• Formulation: 5 mM Sodium Phosphate, pH 5.5 + 0.5 mM DTT
• Country of Origin: USA

Purity Confirmation:

This was determined by SDS-PAGE gel and HPLC analysis.

Activity Assay:

Determined by its ability to chemoattract human CD4+ T lymphocytes using a concentration range of 1.0-100.0 ng/ml.

AA Sequence:

Reconstitution Buffer:

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex.

Storage:

This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% HSA) and store in working aliquots at -20°C to -80°C.