Product Description:

• Endotoxin-free
• Animal-derived product free
• Available in Bulk
• Higher Stability in Media
• 10-Fold Higher Potency
• Lyophilized and Carrier Free (CF

Xeno-free GM-CSF^HuXp is produced in a human cell expression system in serum-free, chemically defined media. It has a demonstrated greater stability under cell culture conditions making it ideal for efficient generation of Human Dendritic Cells. (See the preferred, time saving G4 protocol available at www.HumanZyme.com). GM-CSF is a hematopoietic growth factor that stimulates the development of early erythroid megakaryocytic and eosinophilic progenitor cells. It is produced in endothelial cells, monocytes, fibroblasts, and T lymphocytes. Recombinant Human GM-CSF is a 15 to 36 kDa golbular protein consisting of 128 amino acids, containing two intramolecular disulfide bonds and two potential N-linked glycosylation sites.

Technical Specifications:

• Species: Human
• Expression: HEK293 Cell Expressed
• Activity: Typically ≤2 ng/mL EC50
• Purity: >95%
• Endotoxin: <1 EU/µg
• Molecular Mass: 15 to 36 kDa, monomer, glycosylated
• Formulation: 1x PBS

Purity Confirmation:

The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

Activity Assay:

The activity was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line).

Reconstitution Buffer:

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).