Signosis/Human p53-Regulated cDNA Plate Array/AP-0141/1 Ea
Description:
p53 plays a crucial role in maintaining ordered proliferation, growth, and differentiation of normal cells. While p53 normally exists in the cell at a very low level and in a relatively inactive form, in response to cellular stress it accumulates in the nucleus, where it binds to specific DNA sequences. p53 is known to induce or inhibit the expression of more than 100 target genes. Mutations of the p53 gene is the most frequently encountered genetic event in cancer, thereby inactivating p53 and resulting in the disruption of the expression of target genes. Therefore, expression profiling of p53 target genes will facilitate the functional study of p53.Applicable Grid:
List of Applicable Genes
Principle:
Signosis’ proprietary cDNA plate array is a plate-based hybridization profiling analysis for monitoring the expression of dozens of genes through reverse transcription of mRNA into cDNA. Like array analyses, total RNA is first reverse transcribed into cDNA in the presence of biotin-dUTP in the assay. Targeted genes are then specifically captured onto individual wells on a plate, instead of membranes, through a pre-coated gene-specific oligonucleotide. The captured cDNAs are further detected with streptavidin-HRP. Luminescence is reported as relative light units (RLUs) on a microplate luminometer. The expression level of genes is directly proportional to the luminescent intensity.
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Data:
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