Signosis/TF Activation Profiling Plate Array II with Nuclear Extraction Kit/FA-1002-NE/1 Ea
Description:
Transcription factors (TFs) are a group of cellular proteins that play essential roles in regulating gene expression. Often, a specific cellular signal pathway can activate multiple TFs and the expression of a specific gene is under control of multiple TFs. Therefore, monitoring the activation of multiple TFs simultaneously is very important.Different from TF Activation Profiling Plate Array I, Signosis’ TF activation profiling plate array II is used for monitoring96 different TFsincluding NFkB, AP1, HIF1, and p53. Two 96-well plates are provided for the comparison of two samples.Applicable Grid:
List of Applicable TFs:
4
5
6
11
NF-1
Pit
Stat3
ProX1
HIF
SMUC
Principle:
Signosis’ TF Activation Profiling Arrays are used for monitoring the activities of multiple TFs simultaneously. In the technology, a series of biotin-labeled probes are made based on the consensus sequences of TF DNA-binding sites. When the probe mixtures incubate with nuclear extracts, individual probes will find their corresponding TFs and form TF/probe complexes, which can be easily separated from free probes through a simple spin column purification. The bound probes are then detached from the complexes and analyzed through hybridization with a plate, which each well is specifically pre-coated with complementary sequences of the probes.
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