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Thermofisher旗下Gibco培养系列、ABI细胞计数仪及PCR仪、Invitrogen分子生物学试剂及Qubit3.0、Ambion的RNA相关试剂、Molecularprobes试剂、Pierce蛋白相关试剂盒、Nanodrop One/OneC；Parafilm封口膜；Viskase透析袋；Sigma-Aldrich试剂；AccuStandard标准品；Millipore抗体；Glen Research核酸合成试剂；Bio-Rad电泳仪及PCR仪；Nanocs的PEG聚合物；Chromotek羊驼抗体； Polysciences转染试剂；Echelon-inc脂类研究试剂盒； R&DSystems抗体及Elisa试剂盒；Jackson二抗及封闭血清；Biotium荧光染料；NEB内切酶及NGS相关产品；AthensResearch&Technology人类蛋白及抗血清；Illumina二代测试试剂盒；Tocris小分子试剂；TwistDx恒温扩增试剂盒（RPA）；Enzymatics二代测序酶；Novus抗体及Elisa试剂盒；AG scientific潮霉素B、放线菌酮、G-418；Plantmedia植物培养系列及抗体；Agilent2100生物分析仪；Biolog耗材；Biomatik的Elisa试剂盒；Covaris基因打断仪；CST抗体；Dharmacon的siRNA产品。

美国Pfenex公司总部在加利福尼亚的圣迭戈，是一家为生物制药和疫苗开发机构提供临床前cGMP级蛋白质的全球领先供应商。利用公司的核心技术——Pfēnex Expression Technology™在重组蛋白表达方面的应用，使Pfenex能够快速开发和生产高质量的蛋白和蛋白试剂。现Pfenex公司通过旗下的Reagent Proteins分部为全球药物研究和开发机构生产并销售相关蛋白产品。Pfēnex Expression Technology™核心专利技术结合了一个自动启用高通量平行菌株筛选技术，提供前所未有的能快速成功识别表达高可溶性滴度和活性蛋白的菌株。Pfenex公司已正式授权上海权阳贸易有限公司为中国地区首家且独家产品代理经销商。

Salmon thrombin （三文鱼凝血酶/鲑鱼凝血酶）

Catalog Number

SEA-135

Description

Plasma protein from the blood of farmed S. salar

Product Background

Background:- Fibrin gels made from salmon proteins have demonstrated remarkable properties in neuron survival and growth.- Salmon fibrin gels represent the optimal matrix for neuronal cell cultural- Salmon fibrin gels have also properties superior to mammalian gels in an in vitro angiogenesis model. The salmon gels were 60-188% more efficient than human fibrin gels supporting growth of human endothetlial cells. These results indicate that salmon fibrin may be attractive alternate ECM for support of microvascular network formation.- Salmon fibrinogen and thrombin can be used at room temperature- Soft salmon fibrin gels have been shown to promote selection and growth of tumorigenic cellsResearch Targets:- Regenerative Medicine- Spinal Cord Injury- Neural cell regrowth- Pain & CNSInvestigators- Stem Cell Research- Cancer stem cell researchBenefits:- Superior Performance- demonstrated superiority to Matrigel as an extracellular matrix for neuronal cell growth.- Safety from mammalian pathogens- Easy of rehydration & improved thermal properties.For more information on Salmon fibrinogen and thrombin:Sieminski and Gooch. 2004. J Biomater Sci Polym. Ed 15(2):237-42Ju, et al. Biomaterials. 2007 April; 28 (12): 2097-2108Liu J, et al. Nat Mater. 2012 Jul 1;11(8):734-41. doi: 10.1038/nmat3361.Information SheetApplication Note

Category

Miscellaneous Proteins

Formulation

Dialyzed in 200 mM Tris, 3 mM CaCl2, 0.15M NaCl, pH 7.2-7.5.

Reconstitution

Rehydrate with H2O at room temperature or below. Remove the amount to be used that day, and hold on ice. Salmon thrombin retains greater than 80% activity after 24 hours on ice. Aliquot remaining thrombin and freeze at <-60°C

Storage

Freezer (<-10°C). Avoid freeze-thaw cycles

Purity

Affinity chromatography on immobilized heparin, SDS-PAGESourced from salmon shown by certified tests to be negative for fish viruses. Activity tested by spectrophometric analysis of peptide hydrolysis (Chromozyme), and compared to human standard.Filtered to 0.2 u

Activity

≥ 1000 Units/ mg protein

References

US Patent #6,007,811 and 6,861,225. Michaud et al, 2002. Thrombosis Research 107:245-54Sieminski and Gooch. 2004. J Biomater Sci Polym. Ed 15(2):237-42Ju, et al. Biomaterials. 2007 April; 28 (12): 2097-2108Salmon fibrin treatment of spinal cord injury promotes functional recovery and density of serotonergic innervationLiu J, et al. Nat Mater. 2012 Jul 1;11(8):734-41. doi: 10.1038/nmat3361.

ReagentProteins is the Source for proteins.From custom protein production to our over 5,000 catalog proteins, we are a leading supplier of reagent, pre-clinical and cGMP grade proteins to the biopharmaceutical and vaccine development community. We ensure the cost-effective and rapid delivery of high quality recombinant proteins to enable your research and development efforts.

Pfenex公司总部在加利福尼亚的圣迭戈，是一家为生物制药和疫苗开发机构提供临床前cGMP级蛋白质的全球领先供应商。利用公司的核心技术——Pfēnex expression Technology™在重组蛋白表达方面的应用，使Pfenex能够快速开发和生产高质量的蛋白和蛋白试剂。现Pfenex公司通过旗下的Reagent Proteins分部为全球药物研究和开发机构生产并销售相关蛋白产品。 Pfēnex expression Technology™核心专利技术结合了一个自动启用高通量平行菌株筛选技术，提供前所未有的能快速成功识别表达高可溶性滴度和活性蛋白的菌株。 Pfenex公司已正式授权上海权阳贸易有限公司为中国地区首家且独家产品代理经销商，为中国区各大药企和药物研究机构提供优质蛋白产品，助力药物研发

据悉，Pfenex Inc.于美国纽约证券交易所上市，为高科技生物研发公司，拥有重组DNA蛋白质制造平台(Pfenex Expression Technology®)专利，能有效地生产生物依制遴选药品、同等疗效遴选产品以及遴选疫苗。Pfenex的主导遴选药品为PF708，一种Forteo® (特立帕肽)的等效药品，用于治疗骨质疏松，而其新型炭疽疫苗遴选药品Px563L及RPA563则透过优先开发合同而得到美国政府提供资金。此外，Pfenex正与Jazz Pharmaceuticals合作开发血液╱肿瘤产品，其中包括PF743(一种重组天冬酰胺酶)及PF745(一种具有半衰期延长技术的重组天冬酰胺酶)。此外，Pfenex还生产包括Lucentis®及Neulasta®在内的生物类似遴选药品。