More Information Product Details Synonyms Product Type Properties Source/Host Sequence Crossreactivity Formulation Other Product Data Declaration Shipping and Handling Shipping Short Term Storage Long Term Storage Handling Advice Use/Stability Documents MSDS Product Specification Sheet Datasheet

ISG15 Ubiquitin-like Modifier; G1P2; IFI15; IP17; UCRP

Protein

E. coli

Human ISG15 (Accession Nr. P05161) tagged to vinyl sulfone.

Human

Liquid. In 50mM Hepes pH 6.5, 200mM NaCl, 10 % glycerol.

Use: Potent and highly specific inhibitor of ISG15-specific isopeptidases (such as UBP43). Blocks the hydrolysis of poly-ISG15 chains on substrate proteins in vitro and thus enhances poly-ISG15 chain accumulation. Add directly to in vitro assay from the stock solution. Depending on conditions, typical concentrations to fully inhibit ISG15 deconjugating enzymes in vitro are 1-5μM.

Manufactured by Boston Biochem

DRY ICE

-20°C

-80°C

Aliquot to avoid freeze/thaw cycles.

Stable for at least 1 year after receipt when stored at -80°C.

No

Download PDF

Interferon-stimulated Gene 15 (ISG15), also known as UCRP, is a ubiquitin-like protein that is covalently coupled to target proteins in a process termed ISGylation. It is a 165 amino acid (aa) polypeptide with a predicted molecular weight of 18 kDa. ISG15 exhibits 66% aa sequence identity with its mouse ortholog. Structurally, ISG15 consists of two tandem ubiquitin-like domains that share a similar 3-dimensional structure with ubiquitin and other ubiquitin-like modifiers including NEDD8 and SUMO1. Modification of targets by ISG15 occurs in a stepwise enzymatic process similar to that of ubiquitin. Enzymes regulating ISGylation include the activating (E1) enzyme UBE1L, the conjugating (E2) enzyme UbcH8, and ligases (E3) such as TRIM25 and HERC5. Removal of ISG15 is catalyzed by the deconjugating enzyme UBP43/USP18. Functionally, ISG15 has putative roles in the immune response and tumorigenesis. This is reflected by intracellular ISG15 targets that include Cyclin D1, tumor suppressor p63, IRF3 and a range of viral proteins. It is induced by type 1 interferons and microbial infection and knockout mice exhibit an increased sensitivity to infection by some viruses. ISG15 can also be secreted by cells of the immune system and may act in a cytokine-like manner. For instance, it is produced by human granulocytes in response to mycobacterium exposure and natural killer cells and T cells respond to extracellular ISG15 with IFN-γ production. Further supporting a role in immune function, ISG15 mutations are associated with MSMD, an inherited disorder characterized by increased susceptibility to mycobacterial infection.