R&DSystems公司MAB1410/HumanCrypticMAb(Clone219708)现货低价

25ug

Cryptic,alsoknownatCFC-1,wasoriginallyidentifiedasasecretedproteinthatisspecificallyexpressedduringmesodermdifferentiation(1).Cryptic,alongwithCripto,XenopusFRL-1andzebrafishOEP(one-eyedpinhead)definetheepidermalgrowthfactor-CriptoFRL1Cryptic(EGF-CFC)familyofsignalingproteinsthatfunctionincancerandvariousdevelopmentalprocesses(2, 3).Overallsequenceidentitybetweenmembersofthefamilyislow,buttheydosharedistinctdomains:avariantEGF-likemotif,anovelconservedcysteine-richdomain(calledCFCdomain),andaC-terminalhydrophobicregion(2).MostEGF-CFCmembershaveaglycosyl-phosphatidylinositol(GPI)anchoringsiteattheC-terminusandexistasextracellularmembrane-anchoredproteins.However,naturally-occurringsolubleisoformsalsoexist.HumanCrypticshares55%and25%aminoacididenititywithmouseCrypticandhumanCripto,respectively.Despiteweakconservationinaminoacididentity,EGF-CFCfamilymembersappeartofunctionsimilarlyinassaysforphenotypicrescueofzebrafishoepmutants(2).Crypticisexpressedduringgastrulationinthemesodermandlaterintheneuroectoderm,markingtheProspectivefloorplateoftheneuraltube(1).GeneticevidencefrommiceandhumanspointstoaroleforCrypticindeterminingleft-rightasymmetry.Mutationsinthecrypticgeneresultinaspectrumofheart,lungandspleendefects,allrepresentingleft-rightlateralitydefects(4,5).ThesephenotypesresemblesomeNodalmutantallelessuggestingthatCryptic,likeCripto,actsasanessentialcofactorforNodalsignaling(1,3). Studieshave shownthatotherTGF-beta superfamilymembersinvolvedinmesoderminductionandleft-rightpatterning,Vg1andGDF-1,alsorequireEGF-CFCcofactors.CrypticbindstoGDF-1leADIngtoanActRIIB-ALK4-Cryptic-GDF-1complexforsignaling(6).

• References:

  Showallbackgroundreferences

• Shen,M.etal.(1997)Development124:429.

• Shen,M.andA.Schier(2000)TrendsGenet.16:303.

• Rosa,F.M.(2002)Science\'sSTKEhttp://stke.sciencemag.org/.

• Gaio,U.etal.(1999)Curr.Biol.9:1339.

• Bamford,R.etal.(2000)NatureGenet.26:365.

• Cheng,S.etal.(2003)Genes&Dev.17:31.

• EntrezGeneIDs:

  55997(Human);12627(Mouse)

• AlternateNames:

  CFC1;CFC-1;CFC1B;cripto,FRL-1,crypticfamily1;Crypticfamilyprotein1;crypticprotein;Cryptic;CRYPTICFLJ77897;HTX2MGC133213

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•   1985年，作为公司推出的第一个科研试剂产品，也是全球第一家将该产品进行商业化生产的产品，R&DSystems成功上市了TGF-beta1蛋白。作为胞外信号分子，TGF-beta1蛋白在多种细胞中进行表达，并作为胞外信号分子参与免疫功能，细胞增殖和细胞分化等生物学过程。该产品推出后，公司陆续从生物材料中纯化了几种细胞因子产品并推向市场。