Chimerx/TspGW I/2501/250 u
Details
Source
Purified from E coli strain that carries the cloned tspGWRI gene from Thermus species GW
Unit Definition
One unit is the amount of enzyme required to digest 1µg of pBR322 DNA to obtain a stable digestion pattern in 1 hour in a total reaction volume of 50µl
Reaction Temperature
70°C
Heat Inactivation
No
Assay Unit Substrate
pBR322 DNA
Reaction Buffer
10 mM Tris-HCl (pH 8.5 at 25°C)1 mM dithiothreitol10 mM MgCl2+Enhancers (1)
Notes:
Storage Buffer
10 mM Tris-HCl (pH 7.5 at 25°C)0.1 mM EDTA50 mM NaCl1 mM dithiothrietol100 µg/ml bovine serum albumin50% (v/v) glycerol
Assay Conditions
10 mM Tris-HCl (pH 8.5 at 25°C)10 mM MgCl21 mM dithiothreitol1 µg pBR322 DNAIncubation is at 70°C for 1 hour in a total reaction volume of 50µl
Notes
Storage Conditions
Store at -20°CShipped on dry ice
References
(1) Zylicz-Stachula, A., Harasimowicz-Slowinska, R., Sobolewski, I. and Skowron, P.M. (2002) Nucleic Acids Research 30, 7 e 33
Downloads
Certificate of Analysis PDF - Current Lot
Categories
Restriction Endonucleases
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