Chimerx/Sac I/2350/10,000 u
Details
Sac I Source
Streptomyces achromogenes
Reagents Supplied
CMX Buffer 2
Unit Definition
One unit is the amount of enzyme required to completely digest 1 µg of Adenovirus 2 DNA in 1 hour in a total reaction volume of 50µl
Star Activity
Yes
Incubation Temperature
37°C
Heat Inactivation
10 minutes at 65°C
Assay Unit Substrate
Adenovirus 2 DNA
Reaction Buffer
CMX Buffer 2;10 mM Tris-HCl (pH 7.5 at 37°C) 10 mM MgCl250 mM NaCl1 mM dithiothreitol100 µg/ml bovine serum albumin - See more at: http://www.chimerx.com/store/restriction-endonucleases/hinfi#sthash.eTrsWtsh.dpufCMX Buffer 2:10 mM Tris-HCl (pH 7.5 at 37°C) 10 mM MgCl250 mM NaCl1 mM dithiothreitol100 µg/ml bovine serum albumin
CMX Buffer 2;10 mM Tris-HCl (pH 7.5 at 37°C) 10 mM MgCl250 mM NaCl1 mM dithiothreitol100 µg/ml bovine serum albumin - See more at: http://www.chimerx.com/store/restriction-endonucleases/hinfi#sthash.eTrsWtsh.dpufStorage Buffer
20 mM Tris‑HCl (pH 8.0 at 4ºC) 150 mM KCl500 µg/ml bovine serum albumin0.1% Triton X-100™50% (v/v) glycerol
Assay Conditions
10 mM Tris‑HCl (pH 7.5 at 37ºC)10 mM MgCl2 1 mM dithiothreitol1 µg Adenovirus 2 DNAIncubation is at 37°C for 1 hour in a total reaction volume of 50 µl
Storage Conditions
Store at -20°CShipped on dry ice
Sac I Downloads
Certificate of Analysis PDF-Current LotMSDS PDF-Current Lot
Categories
Restriction endonucleases
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