Details

Source

Haemophilus influenzae Rf

Reagents Supplied

CMX Buffer 2

Unit Definition

One unit is the amount of enzyme required to completely digest 1 µg of lambda DNA in 1 hour at 37°C in a total reaction volume of 50 µl

Star Activity

Yes

Incubation Temperature

37°C

Heat Inactivation

80°C for 10 minutes

Assay Unit Substrate

Lambda DNA

Reaction Buffer

CMX Buffer 2:10 mM Tris-HCl (pH 7.5 at 37°C) 10 mM MgCl₂50 mM NaCl1 mM dithiothreitol100 µg/ml bovine serum albumin

Activity in Buffers

Buffer 1: 25%Buffer 2: 100%Buffer 3: 75%Buffer 4: 75%Buffer 5: 50%*Star activated

Storage Buffer

50 mM Tris-HCl (pH 7.5 at 4°C)0.1 mM EDTA10 mM β-mercaptoethanol500 µg/ml bovine serum albumin50% (v/v) glycerol

Assay Conditions

10 mM Tris‑HCl (pH 7.5 at 37°C)10 mM MgCl₂50 mM NaCl1 mM dithiothreitol100 µg/ml bovine serum albumin1 µg lambda DNAReaction volume of 50 µl

Storage Conditions

Store at -20°CShipped on dry ice

Downloads

Certificate of Analysis PDF-Current LotMSDS PDF-Current Lot