Details

Ssp I Source

Sphaerotilus species

Reagents Supplied

CMX Buffer 2

Unit Definition

One unit is the amount of enzyme required to completely digest 1 µg of lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl

Star Activity

Yes

Reaction Temperature

37°C

Heat Inactivation

10 minutes at 65°C

Assay Unit Substrate

Lambda DNA

Reaction Buffer

CMX Buffer 2:10 mM Tris-HCl (pH 7.5 at 37°C)10 mM MgCl₂50 mM NaCl1 mM dithiothreitol

Activity in Buffers

Buffer 1:50%Buffer 2:100%Buffer 3: 50%Buffer 4: 50%Buffer 5: 50%*Star activity

Storage Buffer

10 mM Tris-HCl (pH 7.4 at 4°C)50 mM KCl0.1 mM EDTA1 mM dithiothreitol500 µg/ml bovine serum albumin0.2% Triton X-100™50% (v/v) glycerol

Assay Conditions

10 mM Tris‑HCl (pH 7.5 at 37°C)10 mM MgCl₂50 mM NaCl1 mM dithiothreitol100 µg/ml bovine serum albumin0.025% Triton X-100™1 µg lambda DNAReaction volume 50 µl

Storage Conditions

Store at -20°CShipped on dry ice

Downloads

Certificate of Analysis PDF - Current LotMSDS PDF-Current Lot

Categories

Restriction Endonucleases