- Description
- Additional
Description
Details
Description:Mouse monoclonal antibody to human interleukin 8 (IL-8)
Purification: Protein G affinity purified
Product Type:Primary antibody, detection antibody in matched antibody pair
Target Protein:Human IL-8
Immunogen:Purified recombinant human IL-8
Fusion Myeloma:P3X63Ag8.653
Specificity:This antibody recognizes both recombinant and native human IL-8.
Species Reactivity: Human, others not tested.
Cross-reactivity:This antibody does not cross react with human Monocyte Chemotactic Activating Factor (MCAF) or RANTES (Regulated on Activation, Normal T-cell Expressed, and Secreted).
Host / Isotype: Mouse, IgG1 Kappa
Formulation: Lyophilized in 0.01M PBS, pH 7.0.
Reconstitution:Double distilled water is recommended and to adjust the final concentration to 1.00 mg/mL.
Storage: Store at -20oC
Research Area:Cytokine, interleukin and chemotaxis
Background:
Interleukin 8 (IL-8), like IL-6, is secreted by macrophages and a variety of cells that express Toll-like receptors in response to the stimulation of pathogens. IL-8"s primary function is to recruit neutrophils and other target cells through chemotaxis to the infected site to eliminate pathogens. IL-8 causes increased intracellular Ca2+, release of reactive oxygen species, and other physiological changes required for migration and phagocytosis. IL-8 is also known to promote angiogenesis.
Applications:
1. ELISA: This antibody can be used as detection antibody in sandwich ELISA applications for human IL-8 detection in combination with a monoclonal capture antibody (Cat No: MO-C40017A).
2. Neutralizing: In chemotaxis assay, the antibody inhibited 98.4% of the chemotactic activity of IL-8 on RB/293 cell using 5mg/mL of antibody and 10ng/mL of human IL-8 in assay. This antibody inhibited the chemotactic effect of human IL-8, had no inhibitory effect to MIP 1-bata and RANTES in neutrophil chemotaxis assay, indicating that the antibody’s neutralization activity is specific. The antibody also neutralized IL-8 induced calcium influx in human granulocytes.
3. Western Blot:The antibody used at 0.02-0.1μg/mL concentrations will allow visualization of 100ng/lane of recombinant human IL-8.
Figure: Western blot analysis of recombinant human IL-8 using clone I8-60.
4.IHC: The antibody was used to detect IL-8 expression in the stratum corneum of psoriatic skin tissue. The image below shows the result of detecting with anti-IL-8 clone 60, biotinylated 2rd antibody and avidin-HRP. The specimen was stained with DAB substrate for 5~10 min, and counter-stained with hematoxylin.
References:
If research is published using this product, please inform Anogen in order to cite the reference on this datasheet. Anogen will provide one unit of product in the same category as gratitude.
Additional
Additional Information
| Product Specificity | mAb anti-Human IL-8, I8-60, Detector |
|---|---|
| Application | ELISA, NT, WB, IHC |
| Size | 0.1 mg |
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
至于到底好不好转就跟你的细胞有关了，和细胞是否是稳转株关系不大。
推荐了解“主细胞库”“工作细胞库”这两个名词。
推荐了解“主细胞库”“工作细胞库”这两个名词。
如果经费充足的话可以找公司包装病毒，如武汉的普健可以提供各种载体的构建及细胞株构建的技术服务。
然后你得确定，这个细胞株对于你得研究来说不可或缺，具有明确的代表性。
上面两个都没有问题的话，祝你顺利。
