
Description
FreeStyle™293ExpressionMediumisananimalorigin–free,chemicallydefined,protein-freemediumspecificallydevelopedtosupportthegrowthandtransfectionof293-FcellsinsUSPensionwithoutadaptation.Themediumiscompleteandready-to-use,withnosupplementationrequired.Gibco®FreeStyle™293ExpressionMediumfeatures:•Completeandready-to-useformatcontainingGlutaMAX™supplement
•ABIlitytosupportsuspensiongrowthandtransfectionof293-Fcells
•Animalorigin–free,protein-freeandchemicallydefinedformulation
•EasyusewiththeFreeStyle™293Expressionsystems
•Scalabilityinspinnerflasksandbioreactors
Completeandready-to-useformatcontainingGlutaMAX™supplement
Gibco®FreeStyle™293ExpressionMediumisreadytouse.Itdoesnotrequiretheadditionofserum,glutamine,orsurfactants.Gibco®FreeStyle™293ExpressionMediumcontainsGlutaMAX™supplementtominimizetoxicammoniabuild-upandimprovecellviabilityandgrowthinaneasy-to-useformat.
Abilitytosupportsuspensiongrowthandtransfectionof293-Fcells
Gibco®293-F,293-H,andFreeStyle™293-FcellsinsuspensioncanbecultureddirectlyintoGibco®FreeStyle™293ExpressionMediumwithoutadaptation.Cellsadaptedtoothercommerciallyavailableserum-freemediacanbesubcultureddirectlyintoGibco®FreeStyle™293ExpressionMedium,usuallywithoutanyfurtheradaptation.Cellsusuallyrequireadaptationfromserum-containingformulations.CellsculturedinGibco®FreeStyle™293ExpressionMediumcanbetransfectedusinglipidbasedtransfectionreagents,suchas293fectin™reagentandFreestyle™MAXreagent.
Animalorigin–free,protein-freeandchemicallydefinedformulation
Gibco®FreeStyle™293ExpressionMediumisanimalorigin–free,protein-freeandchemicallydefined,allowingforeasierpurificationofyourproteinofinterest.Gibco®chemicallydefinedmediacontainnoproteins,hydrolysates,orcomponentsofunknowncomposition.
EasyuseaspartoftheFreeStyle™293ExpressionSystemandFreeStyle™MAXExpressionSystemforproteinproduction
TheFreeStyle™293ExpressionSystemisacomplete,suspensioncellculturesystemforgeneratinglargeamountsofmammalianrecombinantprotein(seefigure).TheFreeStyle™293ExpressionSystemcombinesGibco®FreeStyle™293ExpressionMediumwith293fectin™reagent,acationic-lipidformulationdesignedtotransfectsuspensionFreeStyle™293-Fcellsathighefficiency.TheFreeStyle™MAXExpressionSystemisabreakthroughtechnologyforrapidandhigh-yieldmammalianproteinproduction.TheFreeStyle™MAXExpressionSystemcombinesGibco®FreeStyle™Media,FreeStyle™MAXReagent,andeitherFreeStyle™CHO-ScellsorFreeStyle™293-Fcells.
Scalabilityinspinnerflasksandbioreactors
ProteinproductionusingGibco®FreeStyle™293ExpressionMediumcanbescaledupinspinnerflasksorbioreactors.Theappropriatespinnerorimpellerspeedandseedingdensityshouldbeoptimizedforeachsystem.Dependingontheimpellerdesignandspeed,itmaybenecessarytosupplementGibco®FreeStyle™293ExpressionMediumwithadditionalPluronic®F-68toavoidsheerstressintheculture.
Universalbagpackaging
Gibco®universalbagsofferflexIBLepackagingthatallowsyoutointegrateasingle-use,disposablelargevolumemediacontainerwithawidevarietyofautomatedcellcultureinstruments,disposablebioreactorsystems,andsterileconnectionprocesses,minimizingtheneedforcostlycustomization.
Productuse
CustomersusingGibco®FreeStyle™293ExpressionMediuminamanufacturingprocess,whohaveasubmissionwiththeFDA,mayrequestaletterofauthorizationfromustoreferenceourTypeIIDrugMasterFile(DMF).
cGMPmanufacturingandqualitysystem
Gibco®FreeStyle™293ExpressionMediumismanufacturedatacGMP-compliantfacilitylocatedinGrandIsland,NewYork.ThefacilityisregisteredwiththeFDAasamedicaldevicemanufacturerandiscertifiedtoISO13485standards.
Figures


ComparableexpressioninFreeStyleâ„¢293-FandF17Cells

ComparabletransfectionofFreeStyleâ„¢293-FandF17Cells

ProteinproducedfromtransientFreeStyleâ„¢293-FCelltransfection

Specifications
CellLine: | FreeStyle™293-FCells |
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Classification: | AnimalOrigin-Free, ChemicallyDefined, Protein-Free, Serum-Free |
CultureType: | SuspensionCellCulture |
Form: | Liquid |
Glutamine: | GlutaMAX™-I |
ProductLine: | Freestyle™, Gibco® |
ProductSize: | 1,000mL |
SerumLevel: | Serum-Free |
GreenFeatures: | Sustainablepackaging |
ShippingCondition: | RoomTemperature |
Contents&storage
Shippingconditions:Ambient
Shelflife:12monthsfromdateofmanufacture
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要培养293A细胞,请教各位大神,配制好的完全培养基4度能放置多久?
HT培养基添加剂(HT Supplement)为粉剂,经照射灭菌。每个包装可配制成10ml浓缩液(50X),用于杂交瘤细胞的筛选。
用于酵母繁殖和筛选的培养基
SD是含有盐类、微量元素、维生素、氮源(不含氨基酸的酵母氮碱)和葡萄糖的合成基本培养基。
注意:氮源(不含氨基酸的酵母氮碱)是这种培养基用于筛选的关键,LB,YPD这种复合培养基因为用到了酵母提取物,蛋白胨,基本是氮源全营养的,肯定是不能用来做氮源筛选的
大家好,我是一个质粒小菜鸟,最近遇到个奇怪的事情,希望大家帮忙看看,究竟出了什么问题。
我构建了一个氨苄抗性的质粒,这个质粒在固体培养基里长出了单菌落,但是我挑菌在液体培养基里无法扩增出来。固体培养基和液体培养基一起配置的,氨苄浓度一致,唯一不同的是固体培养基里加入了琼脂。为了防止是氨苄的问题,我把这个固体培养基上的菌落挑划了新的板,依然能长出菌落。而同一次配置的液体培养基,也能扩增其他质粒,所以我实在想不出是什么其他问题,希望大家帮帮忙。
琼脂,学名琼胶,英文名(agar),又名洋菜(agar-agar)、海东菜、冻粉、琼胶、石花胶、燕菜精、洋粉、寒天、大菜丝,是植物胶的一种,常用海产的麒麟菜、石花菜、江蓠等制成,为无色、无固定形状的固体,溶于热水。在食品工业中应用广泛,亦常用作细菌培养基。为什么叫琼脂,主要是用海南的麒麟菜或石花菜制作出来的。海南的简称就是琼。琼脂特性:琼脂的最有用特性是它的凝点和熔点之间的温度相差很大。它在水中需加热至95℃时才开始熔化,熔化后的溶液温度需降到40℃时才开始凝固,所以它是配制固体培养基的最好凝固剂。用琼脂配制的固体培养基,可用以进行高温培养而不熔化,在凝固之前接种时,也不致将培养物烫死。因此,琼脂是制备各种生物培养基中应用最广泛的一种凝固剂。琼脂的浓度,通常是液体培养基的1~1.5%。向左转|向右转
各位大神你们好:
我们是医疗器械工厂,只需配制环境监测和水检测的培养基,但是根据2015版药典规定,1105,141页有一句
而在9203,289页
“商品化的成品培养基除了应附有处方和使用说明外,还应注明有效期、贮藏条件、适用性检查试验的质控菌和用途”。
是否可以理解为只要供应商提供以上信息,使用厂家就可以不用做培养基适用性试验了?
如果购买的商品化的成品培养基不做培养基适用性试验,那么医疗器械的微生物检验将大大减少工作量了。


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