1.Warmplatestoroomtemperaturebeforeuse.Coldplatescausesthetopagartosolidifyirregularly.DOnotwarmplatesto37Casthetopagarwilltakeforevertosolidify.2.PrepareTopAgarastheappropriateliquidmediumwith0.7%agar.Keeping100mlbottlesisconvenient.Forphages,uselTopAgar,whichislessrichandyieldsbiggerplaques.3.Melttopagarinthemicrowavecompletely.Allowtheagartoboilafterliquification;incompletelymeltedagarlooksliquid,butisgrainy.4.Allowagartocoolto48C.Storeinthe48Cwaterbathforconvenience;oldagarturnsdarkandshouldbediscarded.Agarwithdrugscannotbestored.5.Addcellsandphagetoa13mmYellow-CappedTubeinthe48Ctemperatureblock.6.Removethecapandadd2.5mlMoltenTopAgar.Donotreplacecap.7.Immediatelyvortexthemixture,flamethetop,anddispensethemixtureontotheappropriateplate.Swirltheplatetoevenlydistributetheagar.8.Allowtheplatetostandforseveralminutesastheagarcools.9.Afterthetopagarissolid,invertplateandincubate.
