
Type: Protein
Species Reactivity: R
B=Bovine; Ca=Cat; Ch=Chicken; D=Dog; EQ=Equine; GP=Guinea Pig; H=Human; M=Mouse; P=Porcine; Pr=Primate; R=Rat; Rb=Rabbit; Y=Yeast; Xe=Xenopus; Ze=Zebrafish; ; ; ; NA-Not Applicable; STP=Step-Tactin Proteins; AllGlial Cell Line-derived Neurotrophic Factor (GDNF) is a recently discovered neurotrophic factor that has been shown to promote the survival of various neuronal subpopulations in both the central as well as the peripheral nervous systems at different stages of their development. Neuronal subpopulations that have been shown to be affected by GDNF include motoneurons, midbrain dopaminergic neurons, Purkinje cells and sympathetic neurons.
Native GDNF, a disulfide-linked homodimeric glycoprotein, is a novel member of the TGF-β superfamily. Rat GDNF cDNA encodes a 211 amino acid residue prepropeptide that is processed to yield a dimeric protein composed of two 134 amino acid residue subunits. The GDNF sequence contains two potential glycosylation sites and insect cell-expressed recombinant rat GDNF proteins are glycosylated. Mature rat and human GDNF exhibit approximately 93% amino acid sequence identity and show considerable species cross-reactivity. Cells known to express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes and skeletal muscle cells.
Reference:
A DNA sequence encoding the rat GDNF protein sequence (Lin, L-F. et al 1993, Science 260:1130 - 1132) was inserted in a baculovirus expression vector and expressed in Sf 21 insect cells.
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1、血清:大部分人提及的Hyclone和Gibco,现在很多人对其血清的来源及目前市面上流行的真假好坏有怀疑,有人买到说好,有人说像是假的;也有人用国产的胎牛血清培养的,多提及兰州民海和四季青的,并且对兰州民海似乎支持者较多。对血清的选择还想说,不同厂家不同批次的血清都不同保证成分完全一致,对于使用者而言,可以从外观、培养方面了解血清的质量。
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3、肝素:肝素在内皮细胞培养中的作用是抑制其他杂细胞的生长,尤其是平滑肌细胞的生长,使内皮细胞成为生长优势群,浓度不定,有几种说法:查阅书,15μg/ml;美国ATCC说明书,0.1mg/ml;另有50U/ml,肝素买的话大部分人也都推荐Sigma。
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