TheXpertPhosphataseInhibitorCocktailSolution(100X)
isamixtureofseveralinhibitorstopreventvaluable
proteinsfromdephospholylation.Phosphorylationand
dephosphorylationisamolecularon/offswitchthatregulates
anumberofkeyBIOLOGicalpathwayswithinthecell,
includingsignaltransduction,celldivision,andapoptosis.
TheXpertPhosphataseInhibitorCocktailpreserves
phosphorylationofproteinsincelllysatesandtissue
extracts.Thecocktailcontainsamixtureoffour
phosphataseinhibitorsofbroadspecificity,including
sodiumfluoride,sodiumorthovanadate,sodium
pyrophosphateandsodiumglycerophosphate.
Unlikeothercommerciallyavailablephosphatase
inhibitorcocktailswhichprotectagainsteither
Ser/Thrphosphatasesorproteintyrosine
phosphatases.
TheXpertPhosphataseInhibitorCocktailprotects
phosphoproteinsfrombothfamiliesofphosphatases.
Convenientlypackagedina100Xformat,thecocktail
isstableat4°CandiscompatIBLewithcommonprotein
assays,includingtheBCAProteinAssayandBradford-
basedassayssuchasCoomassie.