| Product Name | Thrombin Receptor Activator for Peptide 6 (TRAP - 6)SFLLRN |
| Size | 5 mg |
| Catalog # | AS-24191 |
| US$ | $50 |
| Purity | % Peak Area By HPLC ≥ 95% |
This peptide forms the N-terminal sequence left after thrombin cleavage of the receptor"s amino-terminal exodomain thereby activating the thrombin receptor PAR1. This sequence then acts as a tethered peptide ligand. Free SFLLRN activates PAR1 independent of receptor cleavage and has been used to probe PAR1 function in various cells and tissues. This peptide is also known to be capable of activating PAR2. | |
| Detailed Information | |
| Storage | -20°C |
| References | 1, Hammes, SR and Coughlin, SR. Biochem, 38:2486-2493 (1999).2, Scarborough, RM. et al. J. Biol. Chem. 267, 13146 (1992).3, Ahmad, S. and PN. Walsh, Biochem. 44, 13858 (2005). 4, Buergler, M. et al. J. Thromb. Thrombolysis 19, 115 (2005). |
| Molecular Weight | 748.9 |
| SFLLRN | |
| Sequence(Three-Letter Code) | H - Ser - Phe - Leu - Leu - Arg - Asn - OH |
| Product Citations | Birukova, A. et al. (2010). Lung endothelial barrier protection by iloprost in the 2-hit models of ventilator-induced lung injury (VILI) involves inhibition of Rho signaling . Transl Res 155, 44. doi:10.1016/j.trsl.2009.09.002.Jenkins, R. et al. (2006). Ligation of protease-activated receptor 1 enhances αv β6 integrindependent TGF-β activation and promotes acute lung injury. J. Clin. Invest 116, 1606. doi:10.1172/JCI27183.Ludeman, M. et al. (2005). PAR1 Cleavage and signaling in response to activated protein C and thrombin. J Biol Chem 280, 13122. doi:10.1074/jbc.M410381200Birukova, AA. et al. (2004). Novel role of microtubules in thrombin-induced endothelial barrier dysfunction. FASEB J 18, 1879. doi: 10.1096/fj.04-2328comKawkitinarong, K. et al. (2004). Differential Regulation of human lung epithelial and endothelial barrier function by thrombin. Am. J. Respir. Cell. Mol. Biol 31(5), 517. doi:10.1165/rcmb.2003-0432OCLudeman, MJ. et al. (2004). Regulated shedding of PAR1 N-terminal exodomain from endothelial cells. J. Biol. Chem 279, 18592. doi: 10.1074/jbc.M310836200 |
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碳酸氢钠溶液是一种co2缓冲液,当瓶内二氧化碳量减少时,碳酸氢钠溶液释放co2,反之吸收co2.因此它能保持瓶内二氧化碳量大致不变.
求采纳为满意回答。
1、 缓冲溶液对反应物的测定没有干扰
2、缓冲组分的浓度为1:1
3、有足够的缓冲容量
4、缓冲溶液的PH应在所需范围内
5、组成缓冲溶液的弱碱PKB和弱酸PKA应接近或等于所需的POH值或PH值(PH+POH=14)
配制
只要知道缓冲对的PH值,和要配制的缓冲液的pH值(及要求的缓冲液总浓度),就能按公式计算[盐]和[酸]的量。这个算法涉及对数换算,较麻烦,前人为减少后人的计算麻烦,已为我们总结出pH值与缓冲液对离子用量的关系并列出了表格。只要我们知道要配制的缓冲液的pH,经查表便可计算出所用缓冲剂的比例和用量。例如配制500nmpH5.8浓度为0.1M磷酸缓冲液。
经查表知pH5.8浓度为0.2M Na2HPO48.0毫升,而0.2M Na2HPO492.0毫升。依此可推论出配制100ml0.1M的磷酸缓冲液需要0.1M Na2HPO48.0毫升,而0.1M Na2HPO4需要92.0毫升。
计算好后,按计算结果准确称好固态化学成分,放于烧杯中,加少量蒸馏水溶解,转移入50ml容量瓶,加蒸馏水至刻度,摇匀,就能得到所需的缓冲液。
各种缓冲溶液的配制,均按表格按比例混合,某些试剂,必须标定配成准确浓度才能进行,如醋酸、氢氧化钠等。另外,所有缓冲溶剂的配制计量都能从以上的算式准确获得。

