
Titanium Taq DNA Polymerase is a blend of a specially engineered Taq, and an antibody for integrated hot-start PCR, which prevents non-specific amplification and primer-dimer formation. Titanium Taq DNA Polymerase is suitable for use in all PCR applications and with a wide range of samples, including bacterial and plasmid DNA, cDNA, and complex genomic DNA. It gives higher PCR product yield than other polymerases, and is active over a wide range of Mg2+ concentrations. Titanium Taq is available in several formats:
Titanium Taq DNA Polymerase is a blend of a specially engineered Taq, and an antibody for integrated hot-start PCR, which prevents non-specific amplification and primer-dimer formation. Titanium Taq DNA Polymerase is suitable for use in all PCR applications and with a wide range of samples, including bacterial and plasmid DNA, cDNA, and complex genomic DNA. It gives higher PCR product yield than other polymerases, and is active over a wide range of Mg2+ concentrations.
Titanium Taq is available in several formats:
- Individual components—separate tubes of Titanium Taq polymerase mix and buffer (dNTPs not included).
- Glycerol-free formulation—50X Titanium Taq SP (Glycerol-Free) is ideal for preparation of custom dried-down PCR master mixes or automation workflows.
- For a complete system, use the Titanium Taq PCR Kit—includes polymerase mix, PCR buffer, 50X dNTP mix, control DNA template, control primer mix, and PCR-grade water.
- A ready-to-use, lyophilized 2X PCR master mix—High Yield PCR EcoDry Premix for convenient room-temperature set up, transport, and storage.
- GMP-grade or GPR-registered versions—for an added level of confidence in our polymerase.
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IgM占血清免疫球蛋白总量的6%,主要存在血管内,是机体受抗原刺激后最先产生的抗体,起“先锋免疫”作用,具有很强的细胞毒活性和细胞溶解活性,由于IgM主要存在在血管内,是抗血管内感染的第一线抗体,对防止败血症的发生有重要作用。
IgG-主力抗体
IgG是初级免疫应答中最持久、最重要的抗体,它仅以单体形式存在。大多是抗菌性、抗毒性和抗病毒抗体属于IgG,它在抗感染中起到主力军作用,它能够促进单核巨噬细胞的吞噬作用(调理作用),中和细菌毒素的毒性(中和毒素)和病毒抗原结合使病毒失去感染宿主细胞的能力(中和病毒)。
IgA-局部抗体
按其免疫功能又分为血清型及分泌型两种。血清型IgA存在于血清中,其含量占总IgA的85%左右。血清型IgA虽有IgG和IgM的某些功能,但在血清中并不显示重要的免疫功能。分泌型IgA存在于分泌液中,如唾液、泪液、初乳、鼻和支气管分泌液、胃肠液、尿液、汗液等。分泌型IgA是机体粘膜局部抗感染免疫的主要抗体。故又称粘膜局部抗体。
抗原注射进入动物体内后,因为不是一个单独的分子,会随血液循环输送到各处,也就会接触不同的免疫细胞。有些还会被抗原提呈细胞再加工。所以最后的结果是很多个免疫细胞都会接触到。一个抗原分子也有很多抗原表位。每个表位理论上都有能力激活一个B细胞转化为浆细胞。所以体内最后针对该抗原的必定是多克隆
由于动物个体差异的存在,同一抗原免疫同一种系不同个体的动物,产生的抗体的效价有很大的差异.与动物的年龄和营养状况密切相关.免疫用的动物最好选择适龄的健康雄性动物,雌性动物特别是妊娠动物用于制备免疫抗体则非常不合适,有时甚至不产生抗体.
先饲养观察7天其实就是等于隔离检疫,观察一下兔子的身体状况和是否带有其他传染病,淘汰体质多病的个体,防止在免疫过程中死亡或免疫应答不灵敏而不产生抗体.
一个制造一种专一抗体的浆细胞进行培养,就可得到由单细胞经分裂增殖而形成细胞群,即单克隆。单克隆细胞将合成针对一种抗原决定簇的抗体,称为单克隆抗体。

