- SynonymCD58,LFA3,Ag3
- SourceHuman CD58, His Tag (LF3-H5225) is expressed from human 293 cells (HEK293). It contains AA Phe 29-Arg 215 (Accession # AAH05930).Predicted N-terminus: Phe 29Request for sequence
- Molecular Characterization

This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 22.3 kDa. The protein migrates as 30-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>95% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.

Human CD58, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Immobilized Human CD58, His Tag (Cat. No. LF3-H5225) at 10 μg/mL (100 μL/well) can bindHuman CD2, Fc Tag (Cat. No. CD2-H5258) with a linear range of 5-78 ng/mL (QC tested).

Loaded Human CD2, Fc Tag (Cat. No. CD2-H5258) on Protein A Biosensor, can bind Human CD58, His Tag (Cat. No. LF3-H5225) with an affinity constant of 2.3 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
- BackgroundCD antigen CD58 is also known as Lymphocyte function-associated antigen 3 (LFA-3). CD58 is a cell adhesion molecule expressed on Antigen Presenting Cells (APC), particularly macrophages. CD58 /LFA3 is ligand of the T-lymphocyte CD2 glycoprotein, which binds to CD2 (LFA-2) [3] on T cells and is important in strengthening the adhesion between the T cells and Professional Antigen Presenting Cells. In addition, the LFA-3/CD2 interaction may prime response by both the CD2+ and LFA-3+ cells. Polymorphisms in the CD58 gene are associated with increased risk for multiple sclerosis.
- References
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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抗原注射进入动物体内后,因为不是一个单独的分子,会随血液循环输送到各处,也就会接触不同的免疫细胞。有些还会被抗原提呈细胞再加工。所以最后的结果是很多个免疫细胞都会接触到。一个抗原分子也有很多抗原表位。每个表位理论上都有能力激活一个B细胞转化为浆细胞。所以体内最后针对该抗原的必定是多克隆
由于动物个体差异的存在,同一抗原免疫同一种系不同个体的动物,产生的抗体的效价有很大的差异.与动物的年龄和营养状况密切相关.免疫用的动物最好选择适龄的健康雄性动物,雌性动物特别是妊娠动物用于制备免疫抗体则非常不合适,有时甚至不产生抗体.
先饲养观察7天其实就是等于隔离检疫,观察一下兔子的身体状况和是否带有其他传染病,淘汰体质多病的个体,防止在免疫过程中死亡或免疫应答不灵敏而不产生抗体.
IgM占血清免疫球蛋白总量的6%,主要存在血管内,是机体受抗原刺激后最先产生的抗体,起“先锋免疫”作用,具有很强的细胞毒活性和细胞溶解活性,由于IgM主要存在在血管内,是抗血管内感染的第一线抗体,对防止败血症的发生有重要作用。
IgG-主力抗体
IgG是初级免疫应答中最持久、最重要的抗体,它仅以单体形式存在。大多是抗菌性、抗毒性和抗病毒抗体属于IgG,它在抗感染中起到主力军作用,它能够促进单核巨噬细胞的吞噬作用(调理作用),中和细菌毒素的毒性(中和毒素)和病毒抗原结合使病毒失去感染宿主细胞的能力(中和病毒)。
IgA-局部抗体
按其免疫功能又分为血清型及分泌型两种。血清型IgA存在于血清中,其含量占总IgA的85%左右。血清型IgA虽有IgG和IgM的某些功能,但在血清中并不显示重要的免疫功能。分泌型IgA存在于分泌液中,如唾液、泪液、初乳、鼻和支气管分泌液、胃肠液、尿液、汗液等。分泌型IgA是机体粘膜局部抗感染免疫的主要抗体。故又称粘膜局部抗体。

