- SynonymRPG,Protein G
- SourceRecombinant Protein G, His Tag, also known as Recombinant Staphylococcus Aureus protein G148, was expressed in E.Coli at ACRObiosystems.Request for sequence
- Molecular Characterization

Recombinant Protein G, His Tag is fused with the polyhistidine tag at N-terminus and a single cysteine at C-terminus. The reducing (R) protein migrates as a 29 kDa polypeptide. Protein G Protein can bind to human IgG1 Fc.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>98% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in 100 mM NaH2PO4, pH8.5. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.

Recombinant Protein G, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 98%.

Immobilized Recombinant Protein G, His Tag (Cat. No. RPG-S3140) at 2 μg/mL (100 μL/well) can bind Biotinylated Human IgG1 Fc, Avitag (Cat. No. IG1-H8213) with a linear range of 0.01-0.313 μg/mL (Routinely tested).
- BackgroundProtein G is an immunoglobulin-binding protein expressed in group C and G Streptococcal bacteria much like Protein A but with differing specificities. It is a 65-kDa (G148 protein G) and a 58 kDa (C40 protein G) cell surface protein that has found application in purifying antibodies through its binding to the Fc region. The native molecule also binds albumin, however, because serum albumin is a major contaminant of antibody sources, the albumin binding site has been removed from this recombinant form of Protein G.
- References
- (1)Sjobring U, Bjorck L, Kastern W, et al., 1991, J Biol Chem 266 (1): 399–405.
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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抗原注射进入动物体内后,因为不是一个单独的分子,会随血液循环输送到各处,也就会接触不同的免疫细胞。有些还会被抗原提呈细胞再加工。所以最后的结果是很多个免疫细胞都会接触到。一个抗原分子也有很多抗原表位。每个表位理论上都有能力激活一个B细胞转化为浆细胞。所以体内最后针对该抗原的必定是多克隆
由于动物个体差异的存在,同一抗原免疫同一种系不同个体的动物,产生的抗体的效价有很大的差异.与动物的年龄和营养状况密切相关.免疫用的动物最好选择适龄的健康雄性动物,雌性动物特别是妊娠动物用于制备免疫抗体则非常不合适,有时甚至不产生抗体.
先饲养观察7天其实就是等于隔离检疫,观察一下兔子的身体状况和是否带有其他传染病,淘汰体质多病的个体,防止在免疫过程中死亡或免疫应答不灵敏而不产生抗体.
IgM占血清免疫球蛋白总量的6%,主要存在血管内,是机体受抗原刺激后最先产生的抗体,起“先锋免疫”作用,具有很强的细胞毒活性和细胞溶解活性,由于IgM主要存在在血管内,是抗血管内感染的第一线抗体,对防止败血症的发生有重要作用。
IgG-主力抗体
IgG是初级免疫应答中最持久、最重要的抗体,它仅以单体形式存在。大多是抗菌性、抗毒性和抗病毒抗体属于IgG,它在抗感染中起到主力军作用,它能够促进单核巨噬细胞的吞噬作用(调理作用),中和细菌毒素的毒性(中和毒素)和病毒抗原结合使病毒失去感染宿主细胞的能力(中和病毒)。
IgA-局部抗体
按其免疫功能又分为血清型及分泌型两种。血清型IgA存在于血清中,其含量占总IgA的85%左右。血清型IgA虽有IgG和IgM的某些功能,但在血清中并不显示重要的免疫功能。分泌型IgA存在于分泌液中,如唾液、泪液、初乳、鼻和支气管分泌液、胃肠液、尿液、汗液等。分泌型IgA是机体粘膜局部抗感染免疫的主要抗体。故又称粘膜局部抗体。

