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Novus/Recombinant Human TGF-beta 1 Protein/2 ug/240-B-002
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Novus/Recombinant Human TGF-beta 1 Protein/2 ug/240-B-002
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RecombinantHumanTGF-beta1ProteinSummary

AdditionalInformation
ANewTGF-beta1isAvailable!ThenewproteinisHek293expressed!
DetailsofFunctionality
MeasuredbyitsABIlitytoinhibittheIL-4-dependentproliferationofHT‑2mouseT cells.Tsang, M.et al.(1995)Cytokine7:389.TheED50forthiseffectis0.04-0.2ng/mL.ThespecificactivityofRecombinantHumanTGF-beta1isapproximately2.5x104U/μg,whichiscalibratedagainsthumanTGF‑beta1Standard(NIBSCcode:89/514).
Source
ChineseHamsterOvarycellline,CHO-derivedhumanTGF-beta1proteinAla279-Ser390
Accession#
P01137
N-terminalSequence
Ala279
Structure/Form
Disulfide-linkedhomodimer
Protein/PeptideType
RecombinantProteins
Gene
TGFB1
Purity
>97%,bySDS-PAGEvisualizedwithSilverStainingandquantitativedensitometrybyCoomassie®BlueStaining.
EndotoxinNote
<0.10 eu="" per="" 1="" μg="" of="" the="" protein="" by="" the="" lal="">

Applications/Dilutions

TheoreticalMW
12.8kDa(monomer).Disclaimernote:Theobservedmolecularweightoftheproteinmayvaryfromthelistedpredictedmolecularweightduetoposttranslationalmodifications,posttranslationcleavages,relativecharges,andotherexperimentalfactors.
SDS-PAGE
12kDa,reducingconditions24kDa,non-reducingconditions
Publications
ReadPublicationsusing240-Binthefollowingapplications:
  • Bioassay
    570publications
  • CellCulture
    9publications
  • ELISA(Standard)
    7publications
  • ELISADevelopmet
    1publication
  • ELISAStandard
    1publication
  • InVivo
    16publications
  • Standard
    1publication
  • SurfacePlasmonResonance(SPR
    1publication
  • WesternBlot
    4publications

Packaging,Storage&Formulations

Storage
Useamanualdefrostfreezerandavoidrepeatedfreeze-thawcycles.
  • 12monthsfromdateofreceipt,-20to-70°Cassupplied.
  • 1month,2to8°Cundersterileconditionsafterreconstitution.
  • 3months,-20to-70°Cundersterileconditionsafterreconstitution.
Buffer
Lyophilizedfroma0.2μmfilteredsolutioninAcetonitrileandTFAwithBSAasacarrierprotein.
Purity
>97%,bySDS-PAGEvisualizedwithSilverStainingandquantitativedensitometrybyCoomassie®BlueStaining.
ReconstitutionInstructions
PurifiedrecombinanthumanTGF-beta1isanextremelyhydrophobicproteinthatadheresstronglytosurfaces.Toensurerecovery,reconstituteat20μg/mLinsterile4mMHClcontaining1mg/mLhumanorbovineserumalbumin.

Notes

ThisproductisproducedbyandshipsfromR&DSystems,Inc.,aBio-Technebrand.

AlternateNamesforRecombinantHumanTGF-beta1Protein

  • CEDLAP
  • DPD1
  • latency-associatedpeptide
  • TGFbeta1
  • TGFB
  • TGFB1
  • TGF-beta1protein
  • TGFbeta1
  • TGF-beta1
  • TGFbeta
  • TGF-beta-1
  • transforminggrowthfactorbeta-1
  • transforminggrowthfactor,beta1

Background

TGF-beta1(transforminggrowthfactorbeta1)isoneofthreecloselyrelatedmammalianmembersofthelargeTGF-betasuperfamilythatshareacharacteristiccystineknotstructure(1‑7).TGF-beta1,-2and-3arehighlypleiotropiccytokinesthatareproposedtoactascellularswitchesthatregulateprocessessuchasimmunefunction,proliferationandepithelial-mesenchymaltransition(1‑4).EachTGF-betaisoformhassomenon‑redundantfunctions;forTGF-beta1,micewithtargeteddeletionshowdefectsinhematopoiesisandendothelialdifferentiation,anddieofoverwhelminginflammation(2).HumanTGF‑beta1CDNAencodesa390aminoacid(aa)precursorthatcontainsa29aasignalpeptideanda361aaproprotein(8).Afurin‑likeconvertaseprocessestheproproteintogenerateanN‑terminal249aalatency‑associatedpeptide(LAP)andaC‑terminal112aamatureTGF‑beta1(8,9).Disulfide‑linkedhomodimersofLAPandTGF‑beta1remainnon‑covalentlyassociatedaftersecretion,formingthesmalllatentTGF‑beta1complex(8‑10).CovalentlinkageofLAPtooneofthreelatentTGF‑betabindingproteins(LTBPs)createsalargelatentcomplexthatmayinteractwiththeextracellularmatrix(9,10).TGF‑betaisactivatedfromlatencybypathwaysthatincludeactionsoftheproteaseplasmin,matrixmetalloproteases,thrombospondin1andasubsetofintegrins(10).MaturehumanTGF‑beta1shares100%aaidentitywithpig,dogandcowTGF‑beta1,and99%aaidentitywithmouse,ratandhorseTGF‑beta1.Itdemonstratescross‑speciesactivity(1).TGF‑beta1signalingbeginswithhigh‑affinitybindingtoatypeIIser/thrkinasereceptortermedTGF‑betaRII.Thisreceptorthenphosphorylatesandactivatesasecondser/thrkinasereceptor,TGF‑betaRI(alsocalledactivinreceptor‑likekinase(ALK)‑5),oralternatively,ALK‑1.ThiscomplexphosphorylatesandactivatesSmadproteinsthatregulatetranscription(3,11,12).Contributionsoftheaccessoryreceptorsbetaglycan(alsoknownasTGF‑betaRIII)andendoglin,oruseofSmad‑independentsignalingpathways,allowfordisparateactionsobservedinresponsetoTGF‑betaindifferentcontexts(11).
  1. Derynck,R.andK.Miyazono(2008)ColdSpringHarborLaboratoryPress,29.
  2. Dunker,N.andK.Krieglstein(2000)Eur.J.Biochem.267:6982.
  3. Wahl,S.M.(2006)Immunol.Rev.213:213.
  4. Chang,H.etal.(2002)Endocr.Rev.23:787.
  5. Lin,J.S.etal.(2006)Reproduction132:179.
  6. Hinck,A.P.etal.(1996)Biochemistry35:8517.
  7. Mittl,P.R.E.etal.(1996)ProteinSci.5:1261.
  8. Derynck,R.etal.(1985)Nature316:701.
  9. Miyazono,K.etal.(1988)J.Biol.Chem.263:6407.
  10. Oklu,R.andR.Hesketh(2000)Biochem.J.352:601.
  11. deCaestecker,M.etal.(2004)CytokineGrowthFactorRev.15:1.
  12. Zuniga,J.E.etal.(2005)J.Mol.Biol.354:1052.

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抗体作为生物学上的一个有用工具已经超过一个世纪。 1 9 世 纪 7 0 年 代 , Emil vonBehring 和 Shibasaburo Kitasato⑴最先阐述了免疫球蛋白,从那时起,抗体在研究和商业应用方面的使用取得了前所未有的进展。抗体最初是作为治疗病毒性疾病的天然抗血清来使用的。现今,高度工程化的治疗性抗体已被用于治疗多种危及生命的疾病。这一章将介绍纯化抗体的几种方法,尤其是单克隆抗体的纯化,纯化后的单克隆抗体具有多种用途。本章还将介绍抗体纯化前后的鉴定学方法。本章中,我们实验室产生的数 查看更多>
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Protein purification; actin Overview ACTINThe most abundant muscle and non-muscle cytoskeletal protein. MW 42 kDa, 374/375 amino acids; various isoforms; s 查看更多>
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Joseph SambrookPeter Maccallum Cancer Institute and The University of Melbourne, AustraliaDavid W. RussellUniversity of Texas Southwestern Medical Center, Dall 查看更多>
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GE Healthcare品牌特约代理商/现货 查看更多>
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杆状病毒是一类在自然界中专一性感染节肢动物的DNA病毒,病毒粒子呈杆状,基因组为双链环状DNA分子,DNA以超螺旋形式压缩包装在杆状衣壳内,大小在90~180 Kb之间。目前杆状病毒作为高效、安全的无公害生物虫剂广泛应用于害虫防治。 查看更多>
膜蛋白在生物进程中具有关键性的作用。它们参与细胞与细胞之间、细胞与细胞基质 之 间 的 相 互 联 系 胞 器 及 细 胞 的 形 成 过 程 ,各种离子、代谢产物和蛋白质的跨质膜运辅 i,R N A 的跨核膜运输等。鉴于膜蛋白在多种细胞功能中的重要性,目前针对各种疾病的药物约有 5 0 % 都以其为靶点。作者: 伯吉斯等,主译:陈薇,本实验来自「蛋白质纯化指南」 查看更多>
免 疫 球 蛋 白 M(IgM)和 免 疫 球 蛋 白 D(IgD) 的纯化的相关实验步骤、实验技巧、实验protocol、实验经验及常见问题。 查看更多>
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试剂盒的分类组词网123
阿韶flU66E82017-10-03
主要有: 1.磁珠法核酸提取试剂盒
2.磁珠法提取DNA试剂盒
3.DNA提取试剂盒(离心柱法)
4.磁珠法RNA提取试剂盒
5.RNA提取试剂盒(离心柱法)
6.磁珠法植物基因组DNA提取试剂盒
7.反转录试剂盒
8.胶回收试剂盒
9.PCR纯化试剂盒
10.病毒核酸提取试剂盒(磁珠法)
11.土壤基因组DNA提取试剂盒
12.法医样本DNA提取试剂盒(磁珠法)等 1.elisa
常见elisa试剂盒有:
特种蛋白检测elisa试剂盒
如免疫球蛋白,抗链O-aso,类风湿因子RF,C反应蛋白,微量白蛋白,β-2微球蛋白human,铁蛋白,转铁蛋白transferrin等等
肿瘤标志物检测elisa试剂盒:
如肿瘤标志物,组织多肽抗原,肿瘤相关因子,胰腺癌,直肠癌,细胞角蛋白片段,胃肠癌,铁蛋白,糖链抗原,神经特异性稀醇化酶,上皮膜抗原,乳腺癌,人抗小鼠抗体,前列腺,甲胎蛋白,肝癌,甲基苯丙胺,大肠癌,肺癌,大小便隐血检测,癌胚抗原,β-2微球蛋白等等。
食品安全检验elisa试剂盒:
食品中的激素、药物、霉菌毒素、过敏原残留、转基因产品的检测试剂盒,以及微生物、维生素等的检测产品。
植物病毒、细菌、真菌、植物激素和转基因作物的农业诊断试剂盒
动植物疾病诊断类如猪、牛、羊、马等家畜和禽类以及宠物类检测试剂盒
传染病检测elisa试剂盒
如幽门螺杆菌,乙脑,乙肝,丙肝,丁肝,戊肝,庚肝,衣原体,性病,腺病毒,微小病毒B19,天疱疮,水痘-带状疱疹病毒,生殖支原体,伤寒,沙眼,腮腺炎,人型支原体,麻疹,轮状病毒,流行性出血热,淋球菌,莱姆病,柯萨奇,抗解尿支原体,军团菌,结核,胶原,尖锐湿疣,甲肝,脊髓灰质炎,急性胰腺炎尿胰蛋白酶,霍乱,呼吸道合胞病毒,肝吸虫,副流感,肺炎,带状疱疹,传染性单核细胞增多症,层粘蛋白,布鲁氏杆菌,百日咳,白喉,艾柯病毒,EB病毒,A族链球菌等等。
2.免疫共沉淀试剂盒
3.化学发光试剂盒
4.免疫组化试剂盒
5.放射免疫试剂盒
6.免疫荧光试剂盒 1.细胞凋亡试剂盒
2.葡萄糖检测试剂盒
3.支原体检测试剂盒
四、试剂盒使用示例
试剂盒的产生正是为了使实验人员能够摆脱繁重的试剂配制及优化过程,所以试剂盒中一般配备有相应的使用说明书,用户按照说明书不需或只需少量的优化即可得到满意的结果。
⑴试剂盒使用说明书
说明书一般包括公司标志及名称、试剂盒名称、试剂盒组成、保质期、使用领域、使用方法等项目
说明书格式如右图所示:
①一般在页眉页脚处为公司的名称及标志;
②接下来为试剂盒的名称;
③试剂盒组成中应为试剂盒中的所有内容,为了简洁明了,一般以表格的形式表现;
④操作步骤是试剂盒说明书中最重要的部分,内容应准确、简洁、易懂,不能包含带有歧义的句子,更不能含糊其辞,排版上操作步骤应将复杂的步骤拆解,使人一目了然。可以说操作步骤为试剂盒的灵魂。 试剂盒内容(以离心柱型为例):
一般包括:裂解液RL
去蛋白液RW1
漂洗液RW
RNase free ddH2O
吸附柱(RNase free)
过滤柱(RNase free)
缓冲液
离心管(RNase free)
收集管(RNase free)
此外,还配有一份试剂盒使用说明书,根据不同的生产商,可能还配有不同的试剂,如:DNA酶、溶菌酶等。
使用时一定要根据自己的实验需要购买专用提取试剂盒, 如果不是专用试剂盒,或许能提出RNA,但不能确保其质量以及完整性,会影响RT-PCR、Northern blot、Dot blot、Real time RT PCR、芯片分析、polyA 筛选、体外翻译、RNase 保护分析、分子克隆等后续实验的结果。
当前提取效果好的是QIANGEN公司生产的RNA提取试剂盒,但其售价较高,单次实验费用花费太大,对精度要求不高的实验没有必要购买,当然还有其它的进口试剂盒,但是均存在价格高、订货周期长的问题(如果碰上国内无货的时候,要从国外发货,会等很长一段时间)。普通的提取实验用国产的试剂盒就足以,价格不高,基本上各地均有现货,如天根(目前国内生产的试剂盒中销售面比较广的一种)等,其价格比进口试剂盒要便宜许多,且效果还不错,性价比还可以。
当然,就RNA的提取而言,不一定试剂盒的提取效果就非常好,其实采用一些经典的RNA提取方法,效果也很不错,如:TRIZOL、EDTA等,只是试剂盒使用方便,经典的方法操作复杂一些。向左转|向右转
Agrisera代理(抗体、试剂)这个品牌谁了解?质量如何?国内有没有官方代理呢?agrisera代理(抗体、试剂)这个品牌谁了解?质量如何?国内有没有官方代理呢?
是美国最早实现亲和素纯化二抗商业化的生物公司,同时也是世界上最大的二抗和底物显色系统的生产商。其可以提供高质量的用于分子生物学、免疫学、细胞生物学和体外诊断试剂,其产品有:亲和纯化的抗体和偶联物、ELISA和杂交底物、蛋白检测和纯化试剂盒、核酸标记和检测试剂以及其它辅助试剂等。
最近在了解学习免疫组化,很多问题不太明白,请问免疫组化如何正确选择和使用二抗呢?

想通过亲和层析来纯化抗血小板特异性抗体(抗GPIIb/IIIa抗体和抗GPIbα抗体),能否做到呢?谢谢!

看了下说明书,上面没有具体说。感觉捕获抗体和酶标抗体需要对HCP有不同的结合位点才能形成稳定的抗体-HCP-抗体复合物。不知道有没有战友熟悉,求解惑,谢谢!

我的重组载体带His标签,现想买普洛麦格的镍柱纯化试剂盒!去他们网站找到一个MagneHis蛋白纯化和Hislink蛋白纯化系统,看说明书前面一个需要用到磁力架,后面一个是树脂纯化,不知道效果如何!未找到合适试剂!

从小鼠眼球取血得到的血清ELISA,用双抗夹心法。然后一抗用的是来源于兔抗鼠的抗体,那么二抗应该抗兔还是抗鼠?傻傻分不清楚一抗应该是抗来源还是抗血清呀?(双抗夹心法)求大神指教

请问大家,我的体外转录的RNA想纯化后用于细胞转染。如果该RNA不用试剂盒纯化,只用trzol等纯化行吗?效率高吗?你的具体方法步骤是什麽?望有经验的朋友指导。谢谢:)
问个,一抗二抗结合的问题(elisa)123
星辰之守望者2017-10-03
既然二抗一抗能结合,为啥,ELISA,间接法,还有抗原的事
直接,一抗二抗结合,显色不就行了
DNA纯化试剂盒与质粒DNA提取试剂盒这2者之间有区别吗?
我的实验是这样的,先纯化质粒,然后做酶切,再纯化DNA。
最后一步其实也可以用切胶回收试剂盒,我怕做不好,所以想选用DNA纯化试剂盒,但不知跟质粒DNA提取试剂盒是否有区别,因为说明书上说可以用Invitrogen的K210001.我搜索看了一席啊,觉得那个是提取质粒的小提啊。所以产生这个疑问,请大侠告知DNA纯化试剂盒与质粒DNA提取试剂盒这两者之间有区别吗?
用哪个公司的DNA纯化试剂盒比较好。谢谢啦!