Highlights
- Quick (15 minute) recovery of large-sized DNA (e.g., genomic, plasmid [BAC/PAC], viral, phage, etc.) from agarose gels.
- Unique column design for low volume (≥ 10 µl) elution of ultra-pure, high-yield DNA.
- Eluted DNA is well-suited for use in endonuclease digestion, sequencing, labeling, PCR, etc.
Description
Applicable For | High-quality, purified DNA is ideal for sequencing ligation reactions, and any other sensitive downstream applications |
---|---|
Elution Volume | ≥ 10 µl |
Equipment | Microcentrifuge |
Purity | High-quality, purified DNA is especially well-suited for sequencing and ligation reactions |
Sample Source | Agarose gels containing large DNA fragments of interest |
Sample storage | Eluted DNA can be used immediately or stored at ≤ -20°C |
Size Range | 50 bp to 200 kb |
Type | Large DNA |
Yield | Up to 10 µg DNA |
“The best advantage about this kit is that it is really quick to make it, and the total DNA recuperated have a good quality and it have a good concentration to follow the experiments.”
-Ingrid M.
“The ADB buffer and higher temperature used melted the gel quicker than previously used kits thus making DNA recovery using this kit more efficient and generally quicker than previously used kits.”
-Kelly-Anne F.
“The kit was very easy to follow and yielded good results. It was more affordable than the Qiagen Gel Extraction kits, but worked just as efficiently. Solid product“
-Thomas B (University of Tennessee, Knoxville)
Read MoreCat # | Name | Size | Price | |
---|---|---|---|---|
C1001-50 | Collection Tubes | 50 Pack | $15.00 | |
D3004-4-4 | DNA Elution Buffer | 4 ml | $10.00 | |
D3004-4-1 | DNA Elution Buffer | 1 ml | $11.00 | |
D4003-2-24 | DNA Wash Buffer (Concentrate) | 24 ml | $33.00 | |
D4001-1-50 | ADB (Agarose Dissolving Buffer) | 50 ml | $33.00 | |
D4001-1-100 | ADB (Agarose Dissolving Buffer) | 100 ml | $63.00 | |
D4003-2-6 | DNA Wash Buffer (Concentrate) | 6 ml | $10.00 | |
C1002-50 | Zymo-Spin IC-XL | 50 Pack | $66.00 | |
C1002-25 | Zymo-Spin IC-XL | 25 Pack | $34.00 |
ebiomall.com
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看了下说明书,上面没有具体说。感觉捕获抗体和酶标抗体需要对HCP有不同的结合位点才能形成稳定的抗体-HCP-抗体复合物。不知道有没有战友熟悉,求解惑,谢谢!
双抗无论是在免疫学还是细胞操作,细胞培养中都不与二抗等同。双抗可以是抗一种抗原的两个抗体,去双抗夹心法。也可以是抗不同类型反应的作用物,如抗凝剂素。一般指两种抗生素。
我的实验是这样的,先纯化质粒,然后做酶切,再纯化DNA。
最后一步其实也可以用切胶回收试剂盒,我怕做不好,所以想选用DNA纯化试剂盒,但不知跟质粒DNA提取试剂盒是否有区别,因为说明书上说可以用Invitrogen的K210001.我搜索看了一席啊,觉得那个是提取质粒的小提啊。所以产生这个疑问,请大侠告知DNA纯化试剂盒与质粒DNA提取试剂盒这两者之间有区别吗?
用哪个公司的DNA纯化试剂盒比较好。谢谢啦!