
Cat.No.152404 | 100 µl antiserum, lyophilized. For reconstitution add 100µlH2O, then aliquot and store at -20°C until use. |
Applications | WB:1 : 500 up to 1 : 1000(see remarks) galleryIP:not tested yetICC:not tested yetIHC:not tested yetIHC-P/FFPE:not tested yet |
Immunogen | Recombinant protein corresponding to AA 1921 to 2222 from rat Ca2+ channel R-type α-1E (UniProt Id: Q07652) |
Reactivity | Reacts with: rat (Q07652), mouse (Q61290).Other species not tested yet. |
Specificity | Specific for Ca2+ channel α-1E. |
Remarks | WB:Due to its large size, this antibody requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels of anamed or NuPage TRIS-acetate gels from Invitrogen.This protein tends to aggregate after boiling, making it necessary to run SDS-PAGE with non-boiled samples. ECL detection is recommended: |
Datasheet | 152_404.pdf |
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想通过亲和层析来纯化抗血小板特异性抗体(抗GPIIb/IIIa抗体和抗GPIbα抗体),能否做到呢?谢谢!
一抗的英语是 resistance No.1,二抗resistance No.2.
我的实验是这样的,先纯化质粒,然后做酶切,再纯化DNA。
最后一步其实也可以用切胶回收试剂盒,我怕做不好,所以想选用DNA纯化试剂盒,但不知跟质粒DNA提取试剂盒是否有区别,因为说明书上说可以用Invitrogen的K210001.我搜索看了一席啊,觉得那个是提取质粒的小提啊。所以产生这个疑问,请大侠告知DNA纯化试剂盒与质粒DNA提取试剂盒这两者之间有区别吗?
用哪个公司的DNA纯化试剂盒比较好。谢谢啦!

