Cat.No.AG-A20c1 | 1ml 50% slurry |
Storage | Store at 2-8 °C, do not freeze |
Applications | IP:yes |
Capacity (DBC 10%)(determined with 1 mg/mlhu IgG1 MAB using a 1 ml column). | ≥70 mg hu IgG/ml resin at residence time 6 min≥50 mg hu IgG/ml resin at residence time 2.4 min≥25 mg hu IgG/ml resin at residence time 1 min |
Flow rate | 0.16 - 1 mL/min (1 mL cartridge)0.83 - 5 mL/min (5 mL cartridge) |
CIP (0.5 M NaOH 100 Cycles) | No significant change in performance after 100 cycles with 0.5 M NaOH (1 cycle = 15 min contact time) use at room temperature. |
Particle size | 45µm |
Formulation | 50 % slurry in PBS containing 1mM EDTA, 0.01% azide |
Shelf life | Stable for 6 months. |
Documents | MSDS.pdf |
Remarks | Since Protein A Agarose cartridges are pressure resistant for up to 3 bar, they can be used with a flow restrictor. Cartridges can be connected in series to enlarge capacity. |
Data sheet | ag-a20c1.pdf |
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一抗的英语是 resistance No.1,二抗resistance No.2.
我的实验是这样的,先纯化质粒,然后做酶切,再纯化DNA。
最后一步其实也可以用切胶回收试剂盒,我怕做不好,所以想选用DNA纯化试剂盒,但不知跟质粒DNA提取试剂盒是否有区别,因为说明书上说可以用Invitrogen的K210001.我搜索看了一席啊,觉得那个是提取质粒的小提啊。所以产生这个疑问,请大侠告知DNA纯化试剂盒与质粒DNA提取试剂盒这两者之间有区别吗?
用哪个公司的DNA纯化试剂盒比较好。谢谢啦!
想通过亲和层析来纯化抗血小板特异性抗体(抗GPIIb/IIIa抗体和抗GPIbα抗体),能否做到呢?谢谢!
液体的一抗加50%甘油,-20度也不会冻结,可保存3-5年;
经常使用时取出一小份,4度可使用半年之久;
粉末状的二抗直接-20或-80度贮藏就行了。