GenCatch TM Plasmid DNA Mini-Prep Kit
Catalog# | size | Price | Quantity | |
---|---|---|---|---|
2160050 | 50 Mini Preps | $45.00 | ||
2160250 | 250 Mini Preps | $185.00 |
Description | GenCatch TM Plasmid DNAMini Preparation provides a simple, fast and cost-effectivemethod to purify plasmid DNA without phenol/chloroformextraction. It is based on binding of DNA to silica-basedmembranes in a chaotropic salt. An average yield of 1-20 ugplasmid DNA can be expected from 1-5 ml overnight bacterialculture. | ||||
Detailed Components | High quality spin columns, collection tubes together with buffers and protocols. | ||||
Features | Fast: Up to 24 samples can be readily prepared in 30 minutes with minimum efforts. Flexible: Mini columns can used in either centrifugation procedure or using a vacuum manifold. Convenient: No phenol extractions or ethanol precipitations required. Yield:4-20μg of DNA from 2ml of culture Quality DNA prepared by this kit is suitable for many downstream molecular manipulations including PCR, ligation, restriction enzyme digestion, transformation and transfection, radioactive and fluorescent sequencing. | ||||
Technical Data | Plasmid DNA ranging from 4 to 12 kb purified from E. coli DH5a with GenCatch Plasmid DNA Miniprep Kit and run on 1% agarose gel. Restriction enzyme digestion of pBluescriptSK plasmid purified by GenCatchPlasmid DNA Miniprep Kit and analyzed on a 1.5% agarose gel. Sequencing result of plasmid pBluescriptSK purified by GenCatchPlasmid DNA Miniprep Kit using ABI 3730xl sequencer. |
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一抗的英语是 resistance No.1,二抗resistance No.2.
我的实验是这样的,先纯化质粒,然后做酶切,再纯化DNA。
最后一步其实也可以用切胶回收试剂盒,我怕做不好,所以想选用DNA纯化试剂盒,但不知跟质粒DNA提取试剂盒是否有区别,因为说明书上说可以用Invitrogen的K210001.我搜索看了一席啊,觉得那个是提取质粒的小提啊。所以产生这个疑问,请大侠告知DNA纯化试剂盒与质粒DNA提取试剂盒这两者之间有区别吗?
用哪个公司的DNA纯化试剂盒比较好。谢谢啦!
想通过亲和层析来纯化抗血小板特异性抗体(抗GPIIb/IIIa抗体和抗GPIbα抗体),能否做到呢?谢谢!
液体的一抗加50%甘油,-20度也不会冻结,可保存3-5年;
经常使用时取出一小份,4度可使用半年之久;
粉末状的二抗直接-20或-80度贮藏就行了。