Active Interleukin 4 (IL4)
BSF1; BCGF1 ;B Cell Stimulatory Factor 1; Lymphocyte stimulatory factor 1; B Cell Growth Factor; Binetrakin; Pitrakinra
- Product No.APA077Hu61
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Caprine
- Ovine
- Equine
- Bovine
- Porcine
- Gallus
- Canine
- Others
- Multi-species
- Pan-species
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- TraitsFreeze-dried powder
- Purity> 95%
- Isoelectric Point9.3
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- UOM10µg50µg200µg1mg5mg
- FOBUS$ 208 For more details, please contact local distributors!US$ 520 For more details, please contact local distributors!US$ 1040 For more details, please contact local distributors!US$ 3120 For more details, please contact local distributors!US$ 7800 For more details, please contact local distributors!
ACTIVITY TEST of the Active Interleukin 4 (IL4)

Interleukin 4 (IL4) is a cytokine that induces differentiation of naive helper T cellsand monocyte. As reported, IL-4 would induce the differentiation of THP-1 cells into dendritic cells and macrophages in vitro. In house data was obtained by the following experiment :THP-1 cells were cultured in RPMI-1640 and stimulated with 2ng/mL IL-4, after 7 days of stimulation, cell bodies enlarged with extending pseudopodia, and vesicular bodies appeared within the cells, which showed a morphological characteristics of dendritic cells and macrophages.<br/>Cell differentiation of THP1 cells after incubation with IL4 (2ng/mL) for 7 days was shown in Figure 1.
USAGE of the Active Interleukin 4 (IL4)
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Interleukin 4 (IL4)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Interleukin 4 (IL4)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
Polyacrylamide Gel Electrophoresis (PAGE) Experiment Service
INCREMENT SERVICES
BCA Protein Quantification KitMolecular Mass Marker for ProteinMonoclonal Antibody Customized ServicePolyclonal Antibody Customized ServiceProtein Activity Test Experiment ServiceElectrophoretic Mobility Shift Assay (EMSA) Experiment ServiceBufferLentivirus Packaging Experiment ServiceAdenovirus Packaging Experiment ServiceReal Time PCR Experimental ServiceSpike RBD Protein (S-RBD)Protein GProtein A
Related products
| Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) |
| RPA077Hu02 | Recombinant Interleukin 4 (IL4) | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPA077Hu61 | Eukaryotic Interleukin 4 (IL4) | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA077Hu61 | Active Interleukin 4 (IL4) | Cell culture; Activity Assays. |
| APA077Hu01 | Active Interleukin 4 (IL4) | Cell culture; Activity Assays. |
| RPA077Hu01 | Recombinant Interleukin 4 (IL4) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA077Hu02 | Polyclonal Antibody to Interleukin 4 (IL4) | WB; IHC; ICC; IP. |
| PAA077Hu06 | Polyclonal Antibody to Interleukin 4 (IL4) | WB; IHC; ICC; IP. |
| PAA077Hu01 | Polyclonal Antibody to Interleukin 4 (IL4) | WB; IHC; ICC; IP. |
| MAA077Hu22 | Monoclonal Antibody to Interleukin 4 (IL4) | WB; IHC; ICC; IP. |
| SEA077Hu | ELISA Kit for Interleukin 4 (IL4) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA077Hu | CLIA Kit for Interleukin 4 (IL4) | Chemiluminescent immunoassay for Antigen Detection. |
| KSA077Hu01 | ELISA Kit DIY Materials for Interleukin 4 (IL4) | Main materials for "Do It (ELISA Kit) Yourself". |
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
我的实验是这样的,先纯化质粒,然后做酶切,再纯化DNA。
最后一步其实也可以用切胶回收试剂盒,我怕做不好,所以想选用DNA纯化试剂盒,但不知跟质粒DNA提取试剂盒是否有区别,因为说明书上说可以用Invitrogen的K210001.我搜索看了一席啊,觉得那个是提取质粒的小提啊。所以产生这个疑问,请大侠告知DNA纯化试剂盒与质粒DNA提取试剂盒这两者之间有区别吗?
用哪个公司的DNA纯化试剂盒比较好。谢谢啦!
看了下说明书,上面没有具体说。感觉捕获抗体和酶标抗体需要对HCP有不同的结合位点才能形成稳定的抗体-HCP-抗体复合物。不知道有没有战友熟悉,求解惑,谢谢!
补充一点的是有些病毒在人群中的感染是很普遍的,比如HCMV.对于这些病毒IgM的检测结果可以推测是否为近期感染.但这一类病毒其检测的临床意义经常是当其再激活感染时.比如:HCMV感染后就在末梢单个核细胞中潜伏起来,对于正常人而言是不致病的,但当出现HIV感染或应用免疫抑制剂时会出现激活感染而且经常导致严重疾患.对于这种再激活感染虽然也有IgM的升高,但是却在现在ELISA技术的检测限以下,因此是检测不到的,这时就必须进行针对其抗原的检测。
液体的一抗加50%甘油,-20度也不会冻结,可保存3-5年;
经常使用时取出一小份,4度可使用半年之久;
粉末状的二抗直接-20或-80度贮藏就行了。


