
- Description
- Additional
Description
Details
Description:Rabbit anti-human Glutathione S transferase polyclonal antibody, protein G affinity purified
Product Type:Primary antibodies
Target Protein:Glutathione S transferase
Immunogen:Human Glutathione S transferase
Specificity:This antibody recognizes human Glutathione S transferase.
Reactivity: Human, others not tested
Host: Rabbit
Formulation:Lyophilized in pH 7.4, 0.01M PBS. To reconstitute, add appropriate volume of dIH2O to reach a final antibody concentration of 1.0 mg/mL.
Storage: Store at -20oC.Avoid repeated freeze/thaw cycles.
Research Area:Life science research
Applications:
A 26kDa band was detected in the Western blotting experiment (as shown on Fig.1) when the antibody was used at a 1:300,000 diluted concentration, and 2.5 μg GST antigen was loaded to the gel. The 26kDa band corresponds to the full length GST protein. Fig.1 (See image): Western blot analysis of GST full length protein (2.5 μg) using anti-GST polyclonal antibody.
References:
If research is published using this product, please inform Anogen in order to cite the reference on this datasheet. Anogen will provide one unit of product in the same category as gratitude.
Additional
Additional Information
Product Specificity | pAb Rabbit anti-Human Glutathione S Transferase (GST) |
---|---|
Application | WB |
Size | 0.1 mg |
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补充一点的是有些病毒在人群中的感染是很普遍的,比如HCMV.对于这些病毒IgM的检测结果可以推测是否为近期感染.但这一类病毒其检测的临床意义经常是当其再激活感染时.比如:HCMV感染后就在末梢单个核细胞中潜伏起来,对于正常人而言是不致病的,但当出现HIV感染或应用免疫抑制剂时会出现激活感染而且经常导致严重疾患.对于这种再激活感染虽然也有IgM的升高,但是却在现在ELISA技术的检测限以下,因此是检测不到的,这时就必须进行针对其抗原的检测。
看了下说明书,上面没有具体说。感觉捕获抗体和酶标抗体需要对HCP有不同的结合位点才能形成稳定的抗体-HCP-抗体复合物。不知道有没有战友熟悉,求解惑,谢谢!
液体的一抗加50%甘油,-20度也不会冻结,可保存3-5年;
经常使用时取出一小份,4度可使用半年之久;
粉末状的二抗直接-20或-80度贮藏就行了。

