Reporter genes are commonly used in cell biology research.
OZ Biosciences offers reporter mRNAs that can be used as controls to study transfection and expression in mammalian cells using a variety of assays. They mimic fully processed mature mRNA.
These mRNAs are produced by in vitro transcription and stabilized at the 5" end by modified nucleotides capping (Cap1). They contain a poly(A) tail at the 3" end and are optimized to yield improved stability and performance.
They are modified with 5-methoxyuridine (5moU replaces U) to reduce innate immune responses.
We also offer unmodified mRNAs -- #MRNA9 (Tomato), #MRNA15 (GFP), #MRNA16 (F-Luc).
We recommend using our transfection reagents RmesFect or RmesFect Stem in order to reach high transfection efficiency and optimum protein expression level.
mRNA transfection provides several advantages :
- No need of nuclear uptake - protein expression directly in cytoplasm
- Faster protein expression than DNA transfection
- No genomic integration
- Protein expression directly related to mRNA quantity
- Perfect for transfecting slowing or non-dividing cells
- Protein expression in a total promoter-independent manner
- Transient transfection: mRNA based expression of proteins sustains for a limited time
- GFP mRNA has been designed to produce high expression level of Green Fluorescent Protein. It is a commonly used direct detection reporter in mammalian cell culture, yielding bright green fluorescence with an emission peak at 509 nm.
- F-Luc mRNA has been designed to produce high expression level of FireFly Luciferase protein. It is commonly used in mammalian cell culture to measure both gene expression and cell viability. It emits bioluminescence in the presence of the substrate, luciferin.
- Tomato mRNA has been designed to produce high expression level of Orange Fluorescent Protein. The detection can be done by fluorescent or confocal microscopy. The produced Tomato has an excitation maximum at 551-557 nm and emission maximum at 579-583 nm. Tomato expression level can also be monitored by fluorescence-activated cell sorter analysis (FACS).
- mCherry mRNA encodes the fluorescent protein, mCherry, which is derived from DsRed, a protein found in Discosoma sp. mCherry is a monomeric fluorophore with a peak absorption at 587 nm and emission at 610 nm. It is photostable and resistant to photobleaching.
- β-galactosidase (β-gal) mRNA, codes for a protein product of the bacterial LacZ gene. β-gal catalyzes the conversion of β-galactosides into monosaccharides. it is a common marker gene used to assess transfection efficiency.
Size: 20 µg, 100µg and 1mg.
For bulk, please contact us: order@ozbiosciences.com
Storage: -80°C
mRNAs ARE SHIPPED ON DRY ICE
Dry ice fee included in shipping cost
Please log in or register to see the prices for your country
The following items are 5 moU modified mRNAs -- if you need unmodified mRNAs, please visit this page.
ebiomall.com
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一抗的英语是 resistance No.1,二抗resistance No.2.
我的实验是这样的,先纯化质粒,然后做酶切,再纯化DNA。
最后一步其实也可以用切胶回收试剂盒,我怕做不好,所以想选用DNA纯化试剂盒,但不知跟质粒DNA提取试剂盒是否有区别,因为说明书上说可以用Invitrogen的K210001.我搜索看了一席啊,觉得那个是提取质粒的小提啊。所以产生这个疑问,请大侠告知DNA纯化试剂盒与质粒DNA提取试剂盒这两者之间有区别吗?
用哪个公司的DNA纯化试剂盒比较好。谢谢啦!
想通过亲和层析来纯化抗血小板特异性抗体(抗GPIIb/IIIa抗体和抗GPIbα抗体),能否做到呢?谢谢!
液体的一抗加50%甘油,-20度也不会冻结,可保存3-5年;
经常使用时取出一小份,4度可使用半年之久;
粉末状的二抗直接-20或-80度贮藏就行了。