
Product Highlights
- Reproducible – consistent results between technical replicates for increased confidence in results
- Robust – reliable, accurate detection of DNA and RNA targets from a broad range of sample types
- Sensitive – reliable quantification of low abundance targets and scarce samples
- Fast – delivers reproducible, accurate assay results in as little as 30 minutes
- Efficient – excellent performance in multiplex assays
- Specific – antibody-mediated hot-start DNA polymerase minimizes non-specific amplification for improved assay sensitivity and reliability
Product Description
The SensiFAST™ Probe Lo-ROX Kit has been developed for fast, highly reproducible real-time PCR and has been validated on all commonly-used real-time instruments that require a low concentration of the passive reference dye ROX. SensiFAST Probe has been formulated for use with dual-labelled probes, including TaqMan®, Scorpions® and molecular beacon probes.
A combination of the latest advances in buffer chemistry and PCR enhancers, together with an antibody-mediated hot-start DNA polymerase, ensures that the SensiFAST Probe Kit produces reliable assay results under fast thermal cycling conditions. Furthermore, SensiFAST Probe has been optimized to deliver excellent results in multiplex assays.
The SensiFAST ProbeLo-ROX Kit has been optimized to deliver optimal performance in tandem with the SensiFAST cDNA Synthesis Kit, which offers fast, unbiased cDNA synthesis, without compromising cDNA yield or coverage.
Applications
- Gene expression analysis
- DNA / RNA target detection
- Copy number variation (CNV) analysis

Introduction to SensiFAST
Overview, features and benefits of the SensiFAST product familyReal-Time PCR Selection Chart
One-step Vs. Two-step real-time RT PCR
A discussion of the pros and cons of each detection strategy.Application Note
SensiFAST™ Probe Lo-ROX Kitebiomall.com






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补充一点的是有些病毒在人群中的感染是很普遍的,比如HCMV.对于这些病毒IgM的检测结果可以推测是否为近期感染.但这一类病毒其检测的临床意义经常是当其再激活感染时.比如:HCMV感染后就在末梢单个核细胞中潜伏起来,对于正常人而言是不致病的,但当出现HIV感染或应用免疫抑制剂时会出现激活感染而且经常导致严重疾患.对于这种再激活感染虽然也有IgM的升高,但是却在现在ELISA技术的检测限以下,因此是检测不到的,这时就必须进行针对其抗原的检测。
看了下说明书,上面没有具体说。感觉捕获抗体和酶标抗体需要对HCP有不同的结合位点才能形成稳定的抗体-HCP-抗体复合物。不知道有没有战友熟悉,求解惑,谢谢!
液体的一抗加50%甘油,-20度也不会冻结,可保存3-5年;
经常使用时取出一小份,4度可使用半年之久;
粉末状的二抗直接-20或-80度贮藏就行了。

