Name

Ultra SYBR Green qPCR Master Mix (2X, with ROX I)

Cat. #

Application

• Gene expression analysis
• Microarray result validation

Specifications

UltraSYBR Mixture (With ROX) provides a convenient real-time PCR specific reagent by intercalater method using SYBR Green I for detection. It is a 2x concentrated premix of GoldStar Taq DNA polymerase, dNTPs, Mg2+, SYBR Green I dye (detection), ROX reference dye and PCR buffer components. The product utilizes an enzyme for hot start, GoldStar Taq DNA polymerase, which confers a significant reduction in non-specific PCR amplification. As the enzyme buffer system is optimized for real time PCR, the product offers high amplification efficiency and high detection sensitivity in real-time PCR.

In light of the fact that the qPCR instruments can vary from user to user, Cowin offers the UltraSYBR Mixture in a range of formulations, each of which has been carefully optimized to confer the best performance according to the make and model of a qPCR machine. Please use the following table as a guide for selecting the UltraSYBR Mixture that will be most compatible with your choice of a particular instrument/model.

Shipping / Storage

Key features

Manual (protocol)

PCR reaction system

Note: The recommended primer concentration for PCR is between 0.1-1.0 µM of each primer. A final primer concentration of 0.2 µM is likely to yield good results. Adjust the primer concentraction as necessary.