![NanoHelix/Direct qPCR Kit [Probe]/Custom/BDQPR-P](images/no_picture.gif)
Direct qPCR Kit [Probe]
- Real-time amplification DIRECTLY from whole blood, animal, and plant tissues
- Skip DNA purification
- Applicable with intercalating dyes and probes
- TIME and COST saving
POC DIAGNOSTIC applications
Products
Cat.No. | Product | Feature | Size |
---|---|---|---|
BDQPR-P | Direct qPCR Kit [Probe] | - | Custom |
- Description
- Data
- Specification
- Documents
Direct qPCR Kit [Probe] is designed for a probe-based qPCR amplification directly from animal tissues, plant tissues, and various clinical samples including whole blood, serum, urine, hairand swab collections without any DNA purification processes. The 2x Direct qPCR Premix [probe] in this kit contains antibody-inhibited Taq DNA polymerase, dNTPs, MgCI2, stabilizer, and unique buffer system to resist various PCR inhibitors of tissue samples.
Application
- Direct and quantitative real-time PCR
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湿转,跑胶结束后能看到Marker,转膜条件:300mA,150min,因为目的蛋白160kd左右所以时间比较久.转完之后pvdf膜上却未看到marker,丽春红染色可隐约看到一个条带,请问有哪位高人晓得原因么
请教大家一个问题,不同厂家cIEF的Marker能混合使用吗?另外,如果我对其它厂家pI值为6.0的marker进行cIEF测定,那测得的pI值会等于6.0吗?谢谢!
连续三次都这样,Marker的泳道的位置有很多亮条带,但Marker本来的条带是不亮的。我做了内参和目的,一张胶上左右两部分分别都加样品和Marker,切开转膜分别孵育,一块儿显影,内参的Marker就不会亮,只有目的那一张Marker会亮,昨天做的借了别人的Marker,显影出来还是有那个条带。
我的目的条带还没有Marker那部分的条带亮,Marker的部分非常亮。
英 [ˈmɑ:kə(r)] 美 [ˈmɑ:rkə(r)]
n.标识,标记; 记号笔,阅卷人; 防守队员; 特征
复数: markers
双语例句
1. Draw your child's outline with a heavy black marker.
用浓黑色的毡笔画出你孩子的轮廓。
2. He put a marker in his book and followed her out.
他在书里夹了一个书签后便随她出去了。
3. He placed a marker where the ball had landed.
他在球落地的地方放了个标记。
4. Step off twenty feet and then place a marker in the ground.
先步测20英尺,再在地上做出标记.
5. Step out ten feet and then put a marker in the ground.
步测十英尺,然后在地上做出标记.
做western时,看着膜上的Marker条带是直的,但是最后显影出来的目的蛋白的条带全部是歪的,这是为什么?两块胶都是这个问题,欲哭无泪呀!!!求高人指点一下,是什么操作不当导致的
已经重复做了4次了,还是曝出9条带。请求各位大神解析解析==
英 [?mɑ:k?(r)] 美 [?mɑ:rk?(r)]n.标识,标记; 记号笔,阅卷人; 防守队员; 特征复数: markers
双语例句
Draw your child's outline with a heavy black marker.用浓黑色的毡笔画出你孩子的轮廓。2. He put a marker in his book and followed her out.
他在书里夹了一个书签后便随她出去了。
3. He placed a marker where the ball had landed.他在球落地的地方放了个标记。
4. Step off twenty feet and then place a marker in the ground.先步测20英尺,再在地上做出标记.
5. Step out ten feet and then put a marker in the ground.步测十英尺,然后在地上做出标记.

