MycoplasmatestsaccordingtotheEuropeanPharmacopoeia(EP)Chapter2.6.7requireasensitivityof10colonyformingunits(CFU)permlsamplevolumeforNAT-basedmethodssuchasPCRinordertoreplacetrADItionalculturemethods.Thissensitivitymustbeaccomplishedaspartoftherobustnesstestingforanyparticularsamplematrix.
Usingvitalmycoplasmaisnotacceptableforthemajorityofcellculturelaboratoriesduetosafetyregulations.Our10CFU™sensitivitystandardscontainnon-vitalmaterialandallowsafeandreliablevalidation.
ThemycoplasmaweregrowninculturemediumasdescribedinEP2.6.7,subsequentlytitratedandplatedonHayflickandFreymediumforCFUdetermination.Themycoplasmawereharvestedintheearlylogarithmicgrowthphasetoensureahighratioofvitaltonon-vitalmycoplasmaandthusalowratioofGU*toCFU.AllstrainswereobtainedfromtheNationalCollectionofTypeCultures(NCTC),UK,andcultivatedinlowpassages.
ScopeofApplication
10CFU™SensitivityStandardsareintendedforvalidatingtherobustnessandsensitivityofNAT-basedmycoplasmatests.
KitComponents
Unitpackage:3vialscontaining10cfuoftheparticularmycoplasmaaswellas3vialsnegativecontrol
Setpackage:2vialsofeachEP2.6.7-listedmycoplasmacontaining10cfu(18vialsintotal)aswellas2vialsnegativecontrol
Thecomponentsarelyophilized.
SpeciesandOrderInformation
- Cat.No.102-1003Mycoplasmaarginini
- Cat.No.102-2003Mycoplasmaorale
- Cat.No.102-3003Mycoplasmagallisepticum
- Cat.No. 102-4003Mycoplasmapneumoniae
- Cat.No.102-5003Mycoplasmasynoviae
- Cat.No.102-6003Mycoplasmafermentans
- Cat.No. 102-7003Mycoplasmahyorhinis
- Cat.No.102-8003Acholeplasmalaidlawii
- Cat.No. 102-9003Spiroplasmacitri
- Cat.No. 102-0002MycoplasmaSet
Evaluation
ApplicationofaPCR-basedmycoplasmatest,e.g.Venor®GeMqEPMycoplasmaDetectionkit
RequiredConsumables
Sterile1.5mlreactiontubes,filtertips,appropriateDNAextractionkit
Requiredlabdevices
Microcentrifuge,microPipettes
ShelfLifeandStorage
Thedateofexpiryisgivenoneachpackage.Theunopenedkitshouldbestoredat+2to+8°C.
Figure–10CFUwerere-sUSPendedin1mlcellculturemedium,200µlwereusedforDNAextractionwiththeVenor®GeMSamplePreparationKit.ThemycoplasmaPCR-testwasperformedwiththeMicrosart®ATMPMycoplasmakitandaCorbettRotorGene®6000cycler.
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英 [?mɑ:k?(r)] 美 [?mɑ:rk?(r)]n.标识，标记； 记号笔，阅卷人； 防守队员； 特征复数： markers
双语例句
Draw your child's outline with a heavy black marker.用浓黑色的毡笔画出你孩子的轮廓。2. He put a marker in his book and followed her out.
他在书里夹了一个书签后便随她出去了。
3. He placed a marker where the ball had landed.他在球落地的地方放了个标记。
4. Step off twenty feet and then place a marker in the ground.先步测20英尺,再在地上做出标记.
5. Step out ten feet and then put a marker in the ground.步测十英尺,然后在地上做出标记.
湿转,跑胶结束后能看到Marker,转膜条件：300mA,150min,因为目的蛋白160kd左右所以时间比较久.转完之后pvdf膜上却未看到marker,丽春红染色可隐约看到一个条带,请问有哪位高人晓得原因么
连续三次都这样，Marker的泳道的位置有很多亮条带，但Marker本来的条带是不亮的。我做了内参和目的，一张胶上左右两部分分别都加样品和Marker，切开转膜分别孵育，一块儿显影，内参的Marker就不会亮，只有目的那一张Marker会亮，昨天做的借了别人的Marker，显影出来还是有那个条带。
我的目的条带还没有Marker那部分的条带亮，Marker的部分非常亮。
英 [ˈmɑ:kə(r)] 美 [ˈmɑ:rkə(r)]
n.标识，标记； 记号笔，阅卷人； 防守队员； 特征
复数： markers
双语例句
1. Draw your child's outline with a heavy black marker.
用浓黑色的毡笔画出你孩子的轮廓。
2. He put a marker in his book and followed her out.
他在书里夹了一个书签后便随她出去了。
3. He placed a marker where the ball had landed.
他在球落地的地方放了个标记。
4. Step off twenty feet and then place a marker in the ground.
先步测20英尺,再在地上做出标记.
5. Step out ten feet and then put a marker in the ground.
步测十英尺,然后在地上做出标记.
请教大家一个问题，不同厂家cIEF的Marker能混合使用吗？另外，如果我对其它厂家pI值为6.0的marker进行cIEF测定，那测得的pI值会等于6.0吗？谢谢！
[英] [ˈmɑ:kə(r)][美] [ˈmɑ:rkə(r)]
n. 标识，标记；记号笔，阅卷人；防守队员；特征
同反义词
同义词
buoy signal memorial
相关例句
1. Draw your child's outline with a heavy black marker.(14.11K)
用浓黑色的毡笔画出你孩子的轮廓。
2. He put a marker in his book and followed her out.(14.11K)
他在书里夹了一个书签后便随她出去了。
3. He placed a marker where the ball had landed.(12.38K)
他在球落地的地方放了个标记。
4. Step off twenty feet and then place a marker in the ground.(17.57K)
先步测20英尺,再在地上做出标记.
5. Step out ten feet and then put a marker in the ground.(16.7K)
步测十英尺,然后在地上做出标记.
6. The post served as a bound - ary marker.(12.1K)
这个哨站用作标界.
7. He lined the ball over the 365 - foot marker.(16.99K)
他击出一个超过365英尺标志的直飞球.
