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TheH-XStablePrestainedProteinLadderisathree-colorproteinstandardwith12pre-stainedproteinscoveringawiderangemolecularweightsfrom10to245kDa.Proteinsarecovalentlycoupledwithabluechromophoreexceptfortworeferencebands(onegreenandoneredbandat25kDaand75kDarespectively)whenseparatedonSDS-PAGE(Tris-glycinebuffer).TheH-XStablePrestainedProteinLadderisdesignedformonitoringproteinseparationduringSDS-polyacrylamidegelelectrophoresis,verificationofWesterntransferefficiencyonmembranes(PVDF,nylon,ornitrocellulose)andforapproximatingthesizeofproteins.TheladderissuppliedingelloADIngbufferandisreadytouse.
AdditionalInformation
ProductName: | H-XStablePrestainedProteinLadder |
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CatalogNo.: | L2021 |
Size: | 6X500µl |
Range: | ~10kDato~245kDa |
NumberofBands: | 12 |
Concentration: | Approximately0.1~0.4mg/mlofeachproteininthebuffer(20mMTris-phosphate,pH7.5at25°C),2%SDS,0.2mMDithiothreitol,3.6MUrea,and15%(v/v)Glycerol). |
RecommendedLoad: | •3μlor5μlperloadingforclearvisualizationduringelectrophoresison15-wellor10-wellmini-gel,respectively •2~3μlperwellforgeneralWesterntransfering. •Applymoreforthicker(>1.5mm)orlargergel. |
StABIlity: | Stableforupto2weeksat25°C Stableforupto3monthsat4°C Forlongtermstorage,storeat-20°C(Recommended) |
PDFDataSheet: | PDFDatasheet |
Image(s): | (Clickimagetoenlarge)![]() |
ShippingMethod: | Roomtemperatureshipping |