Linear selection markers are ideal for cotransfection with any expression vector where stable integration and expression are required. We offer linear selection markers for either hygromycin (Linear Hygromycin Marker) or puromycin(Linear Puromycin Marker) resistance. These markers are short, purified linear DNA fragments comprised of the marker gene, an SV40 promoter, and the SV40 polyadenylation signal.
Linear selection markers are ideal for cotransfection with any expression vector where stable integration and expression are required. We offer linear selection markers for either hygromycin (Linear Hygromycin Marker) or puromycin(Linear Puromycin Marker) resistance. These markers are short, purified linear DNA fragments comprised of the marker gene, an SV40 promoter, and the SV40 polyadenylation signal.
Cotransfection of a linear selection marker achieves a higher number of positive clones than using either a single response vector containing a selection marker or cotransfecting with a circular selection marker. Linear selection markers are cotransfected at a decreased ratio relative to the expression vector (i.e., 20-fold less of the linear marker).
The pTK-Hyg Vector and the pPur Vector may be used to select for stably transformed mammalian cells using hygromycin and puromycin, respectively. When cotransfected with pTRE2 or a pBI vector, pTK-Hyg is especially useful for selecting double-stable Tet-On or Tet-Off Cell Lines. When integrated into the host cell’s genome, pTK-Hyg does not cause unwanted activation of pTRE- and pBI-derived plasmids because the HSV TK promoter lacks an enhancer element.
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英 [?mɑ:k?(r)] 美 [?mɑ:rk?(r)]n.标识，标记； 记号笔，阅卷人； 防守队员； 特征复数： markers
双语例句
Draw your child's outline with a heavy black marker.用浓黑色的毡笔画出你孩子的轮廓。2. He put a marker in his book and followed her out.
他在书里夹了一个书签后便随她出去了。
3. He placed a marker where the ball had landed.他在球落地的地方放了个标记。
4. Step off twenty feet and then place a marker in the ground.先步测20英尺,再在地上做出标记.
5. Step out ten feet and then put a marker in the ground.步测十英尺,然后在地上做出标记.
请教大家一个问题，不同厂家cIEF的Marker能混合使用吗？另外，如果我对其它厂家pI值为6.0的marker进行cIEF测定，那测得的pI值会等于6.0吗？谢谢！
已经重复做了4次了，还是曝出9条带。请求各位大神解析解析==
英 [ˈmɑ:kə(r)] 美 [ˈmɑ:rkə(r)]
n.标识，标记； 记号笔，阅卷人； 防守队员； 特征
复数： markers
双语例句
1. Draw your child's outline with a heavy black marker.
用浓黑色的毡笔画出你孩子的轮廓。
2. He put a marker in his book and followed her out.
他在书里夹了一个书签后便随她出去了。
3. He placed a marker where the ball had landed.
他在球落地的地方放了个标记。
4. Step off twenty feet and then place a marker in the ground.
先步测20英尺,再在地上做出标记.
5. Step out ten feet and then put a marker in the ground.
步测十英尺,然后在地上做出标记.
连续三次都这样，Marker的泳道的位置有很多亮条带，但Marker本来的条带是不亮的。我做了内参和目的，一张胶上左右两部分分别都加样品和Marker，切开转膜分别孵育，一块儿显影，内参的Marker就不会亮，只有目的那一张Marker会亮，昨天做的借了别人的Marker，显影出来还是有那个条带。
我的目的条带还没有Marker那部分的条带亮，Marker的部分非常亮。
湿转,跑胶结束后能看到Marker,转膜条件：300mA,150min,因为目的蛋白160kd左右所以时间比较久.转完之后pvdf膜上却未看到marker,丽春红染色可隐约看到一个条带,请问有哪位高人晓得原因么
