PrimeSTAR HS DNA Polymerase is a novel high-fidelity DNA polymerase that allows high-efficiency amplification of large DNA products (up to 8.5 kb for human genomic DNA; up to 22 kb for lambda DNA). Its excellent performance is achieved by superior proofreading ability due to a robust 3" → 5" exonuclease activity. The antibody-mediated hot-start (HS) feature provides improved specificity, thus reducing background and proving useful during highly demanding cloning and sequencing applications. PrimeSTAR HSis available in flexible and convenient formats:
- Individual components—PrimeSTAR HSDNA polymerase is supplied with a tube of 5X PrimeSTAR Buffer (with Mg2+) and a tube of dNTPs.
- 2X PCR master mix—a premix that includes PrimeSTAR HS DNA Polymerase, reaction buffer, and dNTPs for simple and convenient PCR setup and minimal pipetting steps.
PrimeSTAR HS DNA Polymerase is a novel high-fidelity DNA polymerase that allows high-efficiency amplification of large DNA products (up to 8.5 kb for human genomic DNA; up to 22 kb for lambda DNA). Its excellent performance is achieved by superior proofreading ability due to a robust 3" → 5" exonuclease activity. The antibody-mediated hot-start (HS) feature provides improved specificity, thus reducing background and proving useful during highly demanding cloning and sequencing applications. PrimeSTAR HSis available in flexible and convenient formats:
- Individual Components—PrimeSTAR HSDNA polymerase is supplied with a tube of 5X PrimeSTAR Buffer (with Mg2+) and a tube of dNTPs.
- 2X PCR master mix—a premix that includes PrimeSTAR HS DNA Polymerase, reaction buffer, and dNTPs for simple and convenient PCR setup and minimal pipetting steps.
The fidelity of PrimeSTAR HS DNA Polymerase was calculated by direct sequencing analysis to provide a more realistic estimate of the rate of incorporated errors compared with other commonly used indirect phenotypic analyses such as lacI. Calculation methods such as lacI, which are often used by other enzyme suppliers, are prone to error due to inherent lethal and silent mutations.
PrimeSTAR HS DNA Polymerase also offers excellent amplification efficiency and a fast reaction time due to its extremely high priming efficiency. Highly specific amplifications can be achieved using short annealing times of just 5–15 seconds.
For the most challenging PCR applications, enhanced versions of PrimeSTAR HS are available with the ability to amplify GC-rich sequences easily, even higher fidelity, and for long-range PCR:
- PrimeSTAR HS with GC buffer provides reliable, high-fidelity amplification for the most difficult GC-rich content (75% or greater).
- PrimeSTAR Max products provide the highest fidelity and fastest extension time of any Takara Bio product.
- PrimeSTAR GXL products provide high fidelity plus the ability to amplify long and GC- or AT-rich targets.
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2、电泳问题:如果你核酸定量浓度不低的话就考虑电泳问题,电泳的时候加上1kb的marker,如果marker跑不出来说明你凝胶有问题,一般只要marker跑出来了,DNA浓度又比较高,而没有条带这样的现象很少见,DNA提取比较简单而且相当稳定,本人当时做甲基化提取的DNA有一次拿出来电泳忘了放回冰箱了,结果大夏天的在外面放了一天,心怀忐忑的进行了一次电泳,结果条带依然给力,一点都没有降解.
这个是用于代理授权的,这个可以说是唯一辨别产品真伪的方法,
有证书的企业,销售的产品可信度比较高。