ProductHighlights:
TruSeqStrandedTotalRNAwithRibo-ZeroGlobinkitsdeliveraclearandcomprehensiveviewofthetranscriptomefromblood-derivedRNA,withafast,efficientsequencinglibrarypreparationworkflow.
High-qualitywhole-transcriptomeanalysiswithprecisestrandinformation
ThesekitscouplethebenefitsofRibo-ZeroribosomalRNAreductionchemistrywithRNAsequencing(RNA-Seq)technologyforwhole-transcriptomeanalysisofhuman,mouse,orratsamples.Thistechnologyprovides:
- Uniformcoverageandprecisemeasurementofstrandorientation
- High-confidencediscoveryofalternativetranscripts,antisenseexpression,andallele-specificexpression
- Discoveryacrossbothcodingandmultipleformsofnon-codingRNA
EfficientribosomalRNAandglobinmRNAremovalinasinglestep
Whole-transcriptomeanalysisofblood-derivedRNArequirestheremovaloftwoformsofabundantRNA—ribosomalRNA(bothcytoplasmicandmitochondrial)aswellasglobinmRNA,whichispresentinhighlevelsinwholeblood.
TrADItionalremovalmethodsrequiretwoindependentsteps.Thismeanstheneedforadditionalreagents,alongerworkflow,andmoreinputRNAlost.TruSeqStrandedTotalRNAwithRibo-ZeroGlobinleveragesRibo-ZerochemistrytoefficientlyremovebothformsofabundantRNAinasingle,rapidstep.
Findanup-to-datelistofhigh-throughputautomationvendorswithroboticsystemscompatIBLewiththislibrarypreparationkit
Specifications:
InputQuantity | 0.1–1ughigh-qualitypurifiedtotalRNAfromblood |
ContentSpecifications | CapturescodingRNAplusmultipleformsofnon-codingRNA |
MechanismofAction | Bead-basedrRNAdepletion,CDNAsynthesis,andPCR |
Multiplexing | Low-throughputkits:Poolupto12samples.Orpoolupto24sampleswithsetsAandBtogether,High-throughputkitversion:Prepare96uniquelyindexedsamples |
SystemCompatibility | NovaSeq5000,GenomeAnalyzerIIx,HiSeq2000,NextSeq500,HiSeq2500,NextSeq550,HiSeq3000,HiSeq1000 |
AutomationCapABIlity | LiquidHandlingRobots |
VariantClass | TranscriptVariants,SingleNucleotidePolymorphisms(SNPs),GeneFusions |
SpeciesCategory | Rat,Mouse |
Method | Whole-TranscriptomeSequencing |
Technology | Sequencing |
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便宜点的就是国产的tiangen,效果会差一点点 如果是大样本的全血 用这个也凑合
根据模板的类型分为DNA类PCR试剂盒和RNA类PCR试剂盒
PCR mix厂家:普洛麦格公司,QIAGEN公司,罗氏公司,TAKARA公司.
成品PCR试剂盒厂家:深圳匹基,上海之江,中山达安,上海科华,上海复星.
2、电泳问题:如果你核酸定量浓度不低的话就考虑电泳问题,电泳的时候加上1kb的marker,如果marker跑不出来说明你凝胶有问题,一般只要marker跑出来了,DNA浓度又比较高,而没有条带这样的现象很少见,DNA提取比较简单而且相当稳定,本人当时做甲基化提取的DNA有一次拿出来电泳忘了放回冰箱了,结果大夏天的在外面放了一天,心怀忐忑的进行了一次电泳,结果条带依然给力,一点都没有降解.
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