
ProductHighlights:
High-QualityWhole-TranscriptomeAnalysiswithPreciseStrandInformation
RNA-Seqtechnologyprovidesuniformcoverage,precisemeasurementofstrandorientationandhigh-confidencediscoveryoffeaturessuchasalternativetranscripts,antisenseexpressionandallele-specificexpressionacrossbothcodingandmultipleformsofnoncodingRNA.TheTruSeqStrandedTotalRNAwithRibo-ZeroPlantKitcouplesthebenefitsofTruSeqRNAlibrarypreparationwithRibo-ZeroribosomalRNAreductionchemistry,providingarobustandscalablesolutionforwhole-transcriptomeanalysis.
Findanup-to-datelistofhigh-throughputautomationvendorswithroboticsystemscompatIBLewiththislibrarypreparationkit
EfficientRibosomalRNAReductionAcrossaRangeofPlantSpecies,StudyDesigns
TheTruSeqStrandedTotalRNAwithRibo-ZeroPlantKitenablesrapidandspecificremovalofcytoplasmic,mitochondrialandchloroplastribosomalRNAfromleaf,seed,androottissue.Thekithasbeenvalidatedforuseinmultipleplantspecies,includingArABIdopsisthaliana,rice,andmaize,andwillprovideefficientrRNAremovalinabroadrangeofadditionalspecies.*
*Forinformationonaparticularspeciesofinterest,contactIlluminaTechnicalSupport.
Specifications:
AssayTime | ~6.5hours |
Hands-OnTime | ~3.5hours |
InputQuantity | 10nghigh-qualitygenomicDNA;10–100ngFFPEDNA(dependingonQCresults) |
MechanismofAction | Probehybridization,extension-ligation,andPCR |
Multiplexing | 1–96 |
ContentSpecifications | Designcustomprobestosequencegenomicregionsofinterest.Contentrange:4–650kb |
VariantClass | ShortTandemRepeats(STRs),SingleNucleotidePolymorphisms(SNPs),GeneFusions,LossofHeterozygosity(LOH),SomaticVariants,ChromosomalAbnormalities,GermlineVariants,StructuralVariants,Insertions-Deletions(indels) |
SystemCompatibility | HiSeq2000,MiSeq,NextSeq550,HiSeq3000,HiSeq1000,MiSeqDxinResearchMode,MiniSeq,HiSeq1500,NextSeq500,MiSeqFGxinResearchMode,HiSeq2500,HiSeq4000 |
SpeciesCategory | Other,Bovine,Mouse,Human,Rat |
SpecializedSampleTypes | FFPE,LowInput |
Technology | Sequencing |
AutomationCapability | LiquidHandlingRobots |
Method | AmpliconSequencing,CustomSequencing |
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我分别用3ul、5ul、8ul都试过了,没有任何条带,用别人别的方法提好的DNA做,却能出现结果,不知道是自己提取的DNA浓度不够,还是根本没有提取出来。
不知道谁用过,或者有更好的全血提取NDA的试剂盒推荐呢?
本人刚刚开始做PCR,谢谢各位高手!
各位战友好,因实验要求需要提取人的唾液DNA,但因标本需要在临床采集,无法做到当天就提取DNA,因此天根等漱口水提DNA的试剂盒都不满足实验需求。
之前一直在用艾德莱的唾液DNA保存运输提取试剂盒,但是这段时间下来感觉提取效率太低,而且特别不稳定,经常50%以上的标本都提不出来DNA,或者浓度特别低,完全崩溃的感觉!所以想更换试剂盒或者直接提供唾液DNA的保存液。另国外的这种试剂盒太贵了,因经费问题无法承受,所以希望战友们能够推荐一家效率高些的试剂盒,小女子不胜感激!!
便宜点的就是国产的tiangen,效果会差一点点 如果是大样本的全血 用这个也凑合


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