![Kapa biosystems/KAPA HyperPlus Kits/KK8700/24 adapters x 40 µl each](images/201806/1528235349885350002.jpg)
Description:
KAPASingle-IndexedAdapterKit,SetA+B(30µM)KAPAHyperPlusKits
NGSlibrarypreparation. Evolved.
TheKAPAHyperPluskitprovidesastreamlinedworkflowthatincludesfragmentationandlibrarypreparationinasingletube.Buildingonindustry-leADInglibraryconstructionefficiencies,thisintegratedsolutioncombinesenzymaticfragmentation,similarinqualitytomechanicalshearing,withthespeedandconvenienceoftagmentation-basedworkflows.Benefitsinclude:
- DNAfragmentationandlibraryprepin2.5hours*
- FlexIBLeDNAsampleinputfrom1ng–1µg*
- Reducedbiasandmaximizesequencecoverage*
- PCR-freeworkflows
NEW!KAPADual-IndexedAdapterKitsarenowavailable.FormoreinformationonKAPAAdapterKits,scrolldowntotheOrderingsection,ordownloadtheKAPAAdapterandBeadCalculator.
Downloadour AdapterandBeadCalculator
*Dataonfile.
ForResearchUseOnly.Notforuseindiagnosticprocedures.
![Workflow](images/kapabiosystems/Workflow-256x300.jpg)
KAPAHyperPlusWorkflow
ProductHighlights
Tunableandreproduciblefragmentation
- Adjustlibraryinsertsizesfrom150–800bpbyvaryingfragmentationtime
- ReproducibleinsertsizesacrossarangeofGCcontentandDNAinputamounts
![](images/kapabiosystems/Figure3_Conversion_Rate_Graph_web_r2-300x180.png)
Industryleadinglibraryyields…
- Routinelyachieveconversionrates>80%from≥100nginput*
- SuperiorperformanceacrossarangeofDNAinputamounts*
- HighlibraryyieldsenablePCR-freeworkflowsfromaslittleas50ngstartingmaterial*
…Enablesuperiorsequencingresults
- Higherconversionratesresultsinfeweramplificationcyclesandlowerduplicationrates
- Detectlow-frequencymutationswithhighconfidenceduetogreaterlibrarydiversityandmoreuniformsequencecoverage
Minimalsequencecoveragebias
- Lowersequencebiaswhencomparedtotagmentationandotherenzymaticfragmentationmethods*
- Equivalentperformancetomechanicalshearing*
- Lessbiasleadstomoreuniformsequencingcoverageandreducedsequencingcosts*
*Dataonfile.
RelatedProducts
Areyousequencinglow-input,FFPEorhighqualityDNA? RNA? CheckouttheseKapaNGSproductstoimproveyourworkflowandresults:
![Sample QC](images/kapabiosystems/Icon__FFPE.png)
KAPAhgDNAQuantificationandQCKit
![Library Amplification](images/kapabiosystems/Icon__Library_Amplification.png)
KAPALibraryAmplificationKits
![Library Quantification](images/kapabiosystems/Icon__Quantification.png)
KAPALibraryQuantificationKits
![KAPA Stranded mRNA-Seq Kits](images/kapabiosystems/Icon__RNA.png)
KAPAStrandedmRNA-SeqKits
Applications:
Applications- WholeGenomeSequencing
- WholeExomeSequencing
- TargetedSequencing(custompanels)
- FFPESampleSequencing
KitSpecificationsandContents/Storage:
KitSpecificationsandContents/StorageKitscanbestoredforupto6monthsat-20ºC.
KitscontainKAPAFragEnzyme,KAPAFragBuffer(10X),KAPAFragConditioningSolution,KAPAEndRepair&A-TailingBuffer, KAPAEndRepair&A-TailingEnzyme, KAPALigationBuffer, KAPADNALigase, KAPAHiFiHotStartReadyMixand KAPALibraryAmplificationPrimerMix.
Specifications
- SpecDescription
- CompatiblePlatformlluminaHiSeq®,MiSeq®,NextSeq®
- LibraryTypeDNA
- StartingMaterialgenomicDNA,FFPEDNA,PCRamplicons
- InputAmount1ng–1µg
ebiomall.com
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2、电泳问题:如果你核酸定量浓度不低的话就考虑电泳问题,电泳的时候加上1kb的marker,如果marker跑不出来说明你凝胶有问题,一般只要marker跑出来了,DNA浓度又比较高,而没有条带这样的现象很少见,DNA提取比较简单而且相当稳定,本人当时做甲基化提取的DNA有一次拿出来电泳忘了放回冰箱了,结果大夏天的在外面放了一天,心怀忐忑的进行了一次电泳,结果条带依然给力,一点都没有降解.
普通的手提或者试剂盒提取的土壤基因组DNA由于常常残留有PCR的强烈抑制物如腐殖酸、棕黄酸等杂质造成实验失败,此外,由于采用了剧烈的玻璃珠击打来破裂菌体,常常造成DNA剪切和降解。本公司经过长期研发开发出了具有自主知识产权的土壤基因组DNA,通过专利配方的腐殖酸和棕黄酸去除试剂配合特殊处理的纯化柱,可以最大程度的去除这些杂质,同时加上多次柱漂洗,确保得到的DNA具有极高纯度,此外独特的抽提和裂解体系可以迅速裂解细胞(壁)和灭活细胞内核酸酶,不需要借助玻璃珠破壁,有效保证了基因组DNA的完整性。
产品特点:
1.本公司独有的专利配方和纯化柱能有效去除腐殖酸等杂质。
2.不需要借助玻璃珠破壁,有效保证了基因组DNA的完整性,长度可达30kb -50kb,可直接用于PCR,Southern-blot和各种酶切反应。
3.兼容性强,适用于各种不同的土壤包括淤泥等提取困难的土壤。
4.多步骤去除各种杂质和抑制物,保证了极高纯度,OD260/OD280典型的比值达1.7~1.9。
5.不需要使用有毒的苯酚等试剂,也不需要乙醇沉淀等步骤。
6.快速,简捷,单个样品操作一般可在60分钟内完成。
你找下,杭州昊鑫生物
我分别用3ul、5ul、8ul都试过了,没有任何条带,用别人别的方法提好的DNA做,却能出现结果,不知道是自己提取的DNA浓度不够,还是根本没有提取出来。
不知道谁用过,或者有更好的全血提取NDA的试剂盒推荐呢?
本人刚刚开始做PCR,谢谢各位高手!
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