Overview
The E.Z.N.A.® Soil DNA Kit is formulated to isolate high purity cellular DNA from soil samples typically containing humic acid and other inhibitors of PCR. This kit uses a novel and proprietary method to isolate genomic DNA from a variety of environmental samples without organic extractions.
This kit has been successfully used to isolate DNA from Gram-positive and -negative bacteria, fungi, yeast, and algae that inhabit a range of samples including clay, sandy, peaty, chalky, or loamy soil samples. Isolated DNA can be used for most downstream applications, including PCR, Southern blot, and NGS analysis.
- Reliable – Reproducible DNA purification from a variety of sample sources
- High quality – Ready-to-use DNA eliminating PCR inhibitors using proprietary inhibitor removal technology
- Yield – Efficient purification of DNA from even specialized samples
- Ease of use – Contains glass beads pre-filled in 2 mL vials
Specifications
For Research Use Only. Not for use in diagnostic procedures.
| Features | Specifications |
|---|---|
| Starting Amount | Up to 1 g |
| Starting Material | Soil |
| Yield | Dependent upon sample |
| Elution Volume | 50-100 μL |
| Technology | HiBind® DNA Mini Column |
| Processing Mode | Manual |
| Throughput | 1-24 |
Kit Components
| Item | Available Separately |
|---|---|
| HiBind® DNA Mini Columns | View Product |
| 2 mL Collection Tubes | View Product |
| Disruptor Tubes | View Product |
| SLX-Mlus Buffer | View Product |
| DS Buffer | Call for Pricing |
| P2 Buffer | View Product |
| cHTR Reagent | View Product |
| XP1 Buffer | --- |
| HBC Buffer | View Product |
| DNA Wash Buffer | View Product |
| Elution Buffer | View Product |
Protocol and Resources
Product Documentation & Literature
PROTOCOL
D5625 Soil DNA Kit
SDS
D5625 SDS
QUICK GUIDE
D5625 Quick Guide
SALES SHEET
APPLICATION NOTE
Superior Performance of Omega Bio-tek’s E.Z.N.A.® Soil DNA Kit Over CompanyM’s Soil DNA Isolation Kit for DNA Extraction from Soil Samples
Product Data
DNA purified from soil samples using E.Z.N.A.® Soil DNA Kit has higher and more consistent yield than using a leading competing product.
Figure 1. Comparison of DNA extraction method from soil samples. DNA yield determined with fluorescence-based dye quantification. 50 µL ZymoBIOMICS™ Microbial Community Standard was added to 200 mg soil samples and DNA was extracted using manufacturer’s recommended protocols. DNA was eluted in 100 µL for both manufacturers.
DNA purified from soil samples using E.Z.N.A.® Soil DNA Kit has better PCR performance than using a leading competing product.
Figure 2. Comparison of Ct values. 20 µL SYBR Green qPCR reaction. 50 µL ZymoBIOMICS™ Microbial Community Standard was added to 200 mg soil samples and DNA was extracted using manufacturer’s recommended protocols. DNA was eluted in 100 µL for both manufacturers.
E.Z.N.A.® Soil DNA Kit performs especially better for gram-positive bacteria than a leading competing product.
Figure 3. DNA yield by bacterial classes. DNA yield determined with fluorescence-based dye quantification. 0.5 mL cultured Gram-positive and Gram-negative bacteria were added to corresponding 200 mg soil samples and DNA was extracted using manufacturer’s recommended protocols. DNA was eluted in 100 µL for both manufacturers.
Publications
- Bao, Yun-Juan, et al. “High-Throughput Metagenomic Analysis of Petroleum-Contaminated Soil Microbiome Reveals the Versatility in Xenobiotic Aromatics Metabolism.” Journal of Environmental Sciences, vol. 56, 1 June 2017, pp. 25–35, www.sciencedirect.com/science/article/pii/S1001074216306155?casa_token=QXfnahukMoEAAAAA:H6xUoAHyfOBFs6fE1a0KcdKtiPZ53A_6EwwHMyW2uqsNhyydq52wc2CqS_UZCLFTCMB3hrpJ4yk, 10.1016/j.jes.2016.08.022. Accessed 1 June 2020.
- Bin, Zhang, et al. “Dynamic and Distribution of Ammonia-Oxidizing Bacteria Communities during Sludge Granulation in an Anaerobic–Aerobic Sequencing Batch Reactor.” Water Research, vol. 45, no. 18, 15 Nov. 2011, pp. 6207–6216, www.sciencedirect.com/science/article/pii/S0043135411005458?casa_token=-Dxbvqho-9QAAAAA:yjdHqmoRGVk32viDMqj0l1K-hpelh9RhpMa2Z8PdTweLReQ7xB138QTS4LTnaUJIoLa_BBtHgno, 10.1016/j.watres.2011.09.026. Accessed 1 June 2020.
- Chen, Hong, et al. “Effects of ammonia on anaerobic digestion of food waste: process performance and microbial community.” Energy & Fuels 30.7 (2016): 5749-5757.
- Huang, Lu, et al. “Antibiotic Resistance Genes (ARGs) in Duck and Fish Production Ponds with Integrated or Non-Integrated Mode.” Chemosphere, vol. 168, Feb. 2017, pp. 1107–1114, 10.1016/j.chemosphere.2016.10.096. Accessed 9 Apr. 2020.
- Liu, Chao, et al. “The Effects of PH and Temperature on the Acetate Production and Microbial Community Compositions by Syngas Fermentation.” Fuel, vol. 224, 15 July 2018, pp. 537–544, www.sciencedirect.com/science/article/pii/S0016236118305337?casa_token=G8sFg1-lAY8AAAAA:vc4RvQIHqKFWs7GV4IYgsFbE19hHKG64wJa-VxHX2i4bWFeht1IvIjU2sKH_DqSD7k-vhD60_yE, 10.1016/j.fuel.2018.03.125. Accessed 1 June 2020.
- Liu, Shuang Ping, et al. “Bacterial Succession and the Dynamics of Volatile Compounds during the Fermentation of Chinese Rice Wine from Shaoxing Region.” World Journal of Microbiology and Biotechnology, vol. 31, no. 12, 22 Oct. 2015, pp. 1907–1921, 10.1007/s11274-015-1931-1. Accessed 19 May 2020.
- Luo, Haiping, et al. “Sulfate Reduction and Microbial Community of Autotrophic Biocathode in Response to Acidity.” Process Biochemistry, vol. 54, 1 Mar. 2017, pp. 120–127, www.sciencedirect.com/science/article/pii/S1359511316304482?casa_token=UsEoVQm9jdgAAAAA:K3y18r9pkFNEGbVXRhH9NYO2kn92gUTagQO4W9ne_7__4cREpqqMoMgpy3GTE4TGCsY2GmAOpUc, 10.1016/j.procbio.2016.12.025. Accessed 1 June 2020.
- Qin, Sijun, et al. “Forage Crops Alter Soil Bacterial and Fungal Communities in an Apple Orchard.” Acta Agriculturae Scandinavica, Section B — Soil & Plant Science, vol. 66, no. 3, Oct. 2015, pp. 229–236, 10.1080/09064710.2015.1088569. Accessed 1 June 2020.
- Sun, Zhenli, et al. “Effects of BmCPV Infection on Silkworm Bombyx Mori Intestinal Bacteria.” PLOS ONE, vol. 11, no. 1, 8 Jan. 2016, p. e0146313, 10.1371/journal.pone.0146313. Accessed 1 June 2020.
- Wang, Honglei, et al. “Distribution Patterns of Nitrogen Micro-Cycle Functional Genes and Their Quantitative Coupling Relationships with Nitrogen Transformation Rates in a Biotrickling Filter.” Bioresource Technology, vol. 209, 1 June 2016, pp. 100–107, www.sciencedirect.com/science/article/pii/S0960852416302656?casa_token=cLS57Ina4g8AAAAA:JNbvH3JnbxWoT94kv786jXpCfJCgxl6uJBxSB3lvU4fyXMw4NUfFrn8wCpB6M-PtoKIRRIZqzKc, 10.1016/j.biortech.2016.02.119. Accessed 1 June 2020.
- Wang, Wen, et al. “Enhanced Fermentative Hydrogen Production from Cassava Stillage by Co-Digestion: The Effects of Different Co-Substrates.” International Journal of Hydrogen Energy, vol. 38, no. 17, 10 June 2013, pp. 6980–6988, www.sciencedirect.com/science/article/pii/S0360319913008525?casa_token=Yp5RO0rqkckAAAAA:TQ7GNvTa5EwFEgQpDmocPa28hj0Eq3LeR6esG9BZujRWKeb9Pl8UZnzwX4c64PKz2wRwp5I902E, 10.1016/j.ijhydene.2013.04.004. Accessed 1 June 2020.
- Zhang, Bin, et al. “Microbial Population Dynamics during Sludge Granulation in an Anaerobic–Aerobic Biological Phosphorus Removal System.” Bioresource Technology, vol. 102, no. 3, 1 Feb. 2011, pp. 2474–2480, www.sciencedirect.com/science/article/pii/S0960852410018195?casa_token=xABqGAsRc00AAAAA:3-gK60wIYoio7fBqwmlb8OM2y3fRILBwWlBaemc0pz6_fZFumW9c9gC0xMmpg_gXkfTJspCIl6o, 10.1016/j.biortech.2010.11.017. Accessed 1 June 2020.
- Zhi, Wei, et al. “Enhanced long-term nitrogen removal and its quantitative molecular mechanism in tidal flow constructed wetlands.” Environmental science & technology 49.7 (2015): 4575-4583.
- Zhou, Min, et al. “Evolution and Distribution of Resistance Genes and Bacterial Community in Water and Biofilm of a Simulated Fish-Duck Integrated Pond with Stress.” Chemosphere, vol. 245, 1 Apr. 2020, p. 125549, www.sciencedirect.com/science/article/pii/S0045653519327894?casa_token=fHNPjCLuBx0AAAAA:AgLxYXp1gUk5K-1Y2pEEZAU8BKUOB8t_P2NU_ZgPs7QGg70yoGzdaxrL-SvewC0Wsf7VBeflGec, 10.1016/j.chemosphere.2019.125549. Accessed 1 June 2020.
- —. “Spread of Resistance Genes from Duck Manure to Fish Intestine in Simulated Fish-Duck Pond and the Promotion of Cefotaxime and As.” Science of The Total Environment, vol. 731, 20 Aug. 2020, p. 138693, www.sciencedirect.com/science/article/pii/S0048969720322105, 10.1016/j.scitotenv.2020.138693. Accessed 1 June 2020.
